I recently read an article about executing a WET sequence for water suppression in urine samples. The 90°excitation pulse should be calibrated at the beginning of the data acquisition for each sample. what causes the changes of pulse length between different samples? I've read the book "200 and more nmr experiments" and other manuals but I couldn't find the answer. I'm confused about how to selected a proper resonance in mixture for calibration. Should I choose the water resonance in urine spectra for calibraion? Because other signals were covered by this huge resonance.
Thanks a lot!
Check if somebody has answered this question on NMRWiki QA forum
Did you find this post helpful? |
Similar Threads
Thread
Thread Starter
Forum
Replies
Last Post
[Question from NMRWiki Q&A forum] Longer pulse length for conducting samples?
Longer pulse length for conducting samples?
Hi, my 1H 90 deg pulses are always longer when I work with my samples that are electrically conducting. Is this a/the reason why it is long? Any similar experiences?
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
News from other NMR forums
0
08-04-2012 05:22 AM
1H NMR Metabolomics Study of Human Urine
1H NMR Metabolomics Study of Human Urine
http://i.ytimg.com/vi/sT4XV9_9KYw/default.jpg
1H NMR Metabolomics Study of Human Urine
A presentation of some recent work from the Metabolomics and Bioinformatics & Statistics Groups at the Institute of Food Research, Norwich, UK. See the teams' webpages at: www.ifr.ac.uk and www.ifr.ac.uk This video is a shortened version of a presentation given at "Advances in Metabolic Profiling 2011".
From:BioStatsXtra
Views:195
http://gdata.youtube.com/static/images/icn_star_empty_11x11.gif http://gdata.youtube.com/static/images/icn_star_empty_11x11.gif...
nmrlearner
NMR educational videos
0
07-30-2012 07:42 AM
[Question from NMRWiki Q&A forum] Calibrate HCCH-TOCSY spectrum with reference to HBHACONH
Calibrate HCCH-TOCSY spectrum with reference to HBHACONH
I am facing problems in calibration of my HCCH-TOCSY spectrum in reference to HBHACONH and HCCCONH spectrum. Can somebody tell me how to solve this issue? Can I autoassign my HCCH-TOCSY spectrum using HBHACONH spectrum
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
News from other NMR forums
0
10-02-2011 08:25 AM
The application of micro-coil NMR probe technology to metabolomics of urine and serum
The application of micro-coil NMR probe technology to metabolomics of urine and serum
Abstract Increasing the sensitivity and throughput of NMR-based metabolomics is critical for the continued growth of this field. In this paper the application of micro-coil NMR probe technology was evaluated for this purpose. The most commonly used biofluids in metabolomics are urine and serum. In this study we examine different sample limited conditions and compare the detection sensitivity of the micro-coil with a standard 5 mm NMR probe. Sample concentration is evaluated as a means to leverage the...
Pulse length/power level calculator
Pulse length/power level ? calculator
http://www.spectroscopynow.com/FCKeditor/UserFiles/File/specNOW/HTML%20files/pulselength.htm
Go to BMNRC community to find more info about this topic.
nmrlearner
NMR software
0
09-26-2010 07:59 AM
[NMR paper] 1H NMR studies of reactions of copper complexes with human blood plasma and urine.
1H NMR studies of reactions of copper complexes with human blood plasma and urine.
Related Articles 1H NMR studies of reactions of copper complexes with human blood plasma and urine.
Biochem Pharmacol. 1992 Jan 22;43(2):137-45
Authors: Bligh SW, Boyle HA, McEwen AB, Sadler PJ, Woodham RH
Reactions of the copper complexes Cu(II)Cl2, 2-, and + (where DIPS is 3,5-diisopropylsalicylate and DMP is 2,9-dimethylphenanthroline) with human blood plasma and urine have been studied by 500 MHz 1H NMR spectroscopy, and CD spectroscopy has been used to...
How to calibrate pulse length for urine samples?
Linear Mode
