Hi all, I want to calculate error in ppm from my Hsqc data. My spectrometer yields X1axis, Y1Axis, DX1, DY1, X1ppm, and Y1ppm. I know that DX1 & DY1 are errors in Hz for X1axis & Y1Axis. But how do you convert that to ppm if i'm working in 900 MHz and Sw of 12626.263 HZ. Thank you
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[Question from NMRWiki Q&A forum] hsqc referencing
hsqc referencing
Hi!I need to know how to reference nitrogen 15 from proton in a foldel hsqc experiment...I don't know how to calculate the new 'lowest frequency' from the parameters I have in the processed spectrum...could someone help me?thanks!!
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nmrlearner
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10-24-2013 08:45 PM
1H-15N HSQC, edited by a 1H inversion recovery and observed in the antiphase component (IR-HSQC-AP)
Could someone explain the experimental theory/basis behind 1H-15N inversion recovery filtered HSQC experiment observed in antiphase (IR-HSQC-AP)? I am still learning basic NMR theory, but would like to know about this particular experiment, which was used to detect paramagnetically broadened backbone resonances. Thanks.
Abstract
Biogenesis of iron–sulfur cluster proteins is a highly regulated process that requires complex protein machineries. In the cytosolic iron–sulfur protein assembly machinery, two human key proteins—NADPH-dependent diflavin oxidoreductase 1 (Ndor1) and...
talderson
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09-23-2013 06:01 AM
[Question from NMRWiki Q&A forum] How to extract residue information in case of line broadening (intensity drop ) of HSQC titration of protein - protein interaction data ?
How to extract residue information in case of line broadening (intensity drop ) of HSQC titration of protein - protein interaction data ?
Dear Friends
we have done HSQC gradient titration with targeted proteins , most of cross peaks we observed intensity drop , 30 out of 95 peaks ( 70 percent intensity drop of each cross peak ) , other cross peaks also we observe intensity drop less than 70 percent , we did n"t observe any chemical shift change .( May be it is due to intermediate exchange regime of complex ) .ITC experiment showing 12uM binding constant .AUC experiment result is...
nmrlearner
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05-09-2012 07:38 PM
[NMRpipe Yahoo group] Re: Phasing 1H-15N HSQC Kinetics data from Bruker Instrument
Re: Phasing 1H-15N HSQC Kinetics data from Bruker Instrument
De. DeRose, Thank you for directing me to the "macro edit" pull down in NMRDraw. This, in addition to your comment regarding dwell times, has seemed to solve
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08-10-2011 02:15 AM
[NMRpipe Yahoo group] Re: Phasing 1H-15N HSQC Kinetics data from Bruker Instrument
Re: Phasing 1H-15N HSQC Kinetics data from Bruker Instrument
Hi Nicole, If the first data point in the indirect dimension started at half the dwell time, the phase correction in the indirect dimension should be -p0 -90
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08-06-2011 01:27 AM
[NMRpipe Yahoo group] Phasing 1H-15N HSQC Kinetics data from Bruker Instrument
Phasing 1H-15N HSQC Kinetics data from Bruker Instrument
Hello group! I should mention that my lab specializes in X-Ray Crystallography and my thesis project has me learning NMR. So, I apologize for the perhaps
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