Question from NMRWiki Q&A forum How to adjust the receiver gain in a deuterium oxide protein sample?

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[Question from NMRWiki Q&A forum] How to adjust the receiver gain in a deuterium oxide protein sample?

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11-28-2013, 05:18 PM

nmrlearner

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How to adjust the receiver gain in a deuterium oxide protein sample?

How to adjust the receiver gain in a deuterium oxide protein sample?

I am running a 15N HSQC on a protein sample which is in a 100% deuterium oxide solvent. The command rga in TopSpin gives a value of >10000 for the receiver gain. I understand this is happening because there is no water signal and hence the receiver gain is optimizing the S/N. However, should the experiment be run with that high value or should it be adjusted to a value of a size similar to the one used on water samples?

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