Hi All, I am new to Sparky. I am trying to use NCO, NCACO and NCOCA spectra to get assignment for protein structure. If anybody has proper instruction to do
[Question from NMRWiki Q&A forum] A beginner's guide to protein backbone assignment?
A beginner's guide to protein backbone assignment?
Hi All,
I'm new to protein backbone assignment by NMR, and my protein is about ~37kDa (about 400 residues). We've acquired a series of triple resonance spectra: HNCA, HNCACB, HNCOCACB, HNCOCA, and HNCACO. I also have an HQCS spectrum. While I know how magnetization is transferred in the above mentioned experiment, how do I start assigning my protein? I use NMRpipe to process the spectra and Sparky for assignment.
Thanks a lot!
nmrlearner
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Corrigendum to “BEST-HNN and 2D (HN)NH experiments for rapid backbone assignment in p
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Publication year: 2010
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Source: Journal of Magnetic Resonance
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automated backbone assignment
Can anyone suggest me a most commonly used and worthful software for automated backbone assignment of triple labeled proteins.
Thanks.
sarun
NMR Questions and Answers
2
03-30-2007 03:21 PM
Mars - automatic backbone assignment of proteins
Link to the program
Mars - robust automatic backbone assignment of proteins.
Jung YS, Zweckstetter M. J Biomol NMR. 2004 Sep;30(1):11-23.
Abstract:
MARS is a program for robust automatic backbone assignment of 13C/15N labeled proteins. It can be applied independent of the assignment complexity, it does not require tight thresholds for establishing sequential connectivity or detailed adjustment of these thresholds, it can work with a wide variety of NMR experiments and it is robust against missing chemical shift information. In case of a known 3D structure, residual dipolar...
Help for backbone assignment using NCACO and NCOCA
Linear Mode
