Let me consider a proton experiment done on a 500 MHz spectrometer. The signal coming from the sample has a frequency of approximately 500 MHz. Then the mixer converts the signal down to a signal in the kHz region by subtracing the reference frequency. This seems clear to me. Now, what appens in the second dimension? Let me take a COSY spectrum as an example. Let me call t1 the evolution time in the second dimension. Which is the frequency of the signal in this dimension? Is it in the kHz or in the MHz range? Every book I look at writes the evolution in the second dimension as if it was in the kHz region (proton frequency minus reference frequency), but I don't understand how is this possible because i don't see any machinery that suctracts the reference from the signal in the second dimension.
Thaks in advance
Check if somebody has answered this question on NMRWiki QA forum
Did you find this post helpful? |
Similar Threads
Thread
Thread Starter
Forum
Replies
Last Post
[NMR paper] Nutation frequency modulation on NMR signal of nuclear spins in chemical exchange with solvent water under the BEST conditions.
Nutation frequency modulation on NMR signal of nuclear spins in chemical exchange with solvent water under the BEST conditions.
Related Articles Nutation frequency modulation on NMR signal of nuclear spins in chemical exchange with solvent water under the BEST conditions.
Magn Reson Chem. 2014 Jan 24;
Authors: Yao S, Keizer DW
Abstract
Solvent exchange properties of protein backbone amide protons provide valuable residue-specific information on protein solvent accessibility, structure stability and flexibility and hence are of significant...
[NMR paper] 1H, 15N, and 13C NMR signal assignments of IIIGlc, a signal-transducing protein of Es
1H, 15N, and 13C NMR signal assignments of IIIGlc, a signal-transducing protein of Escherichia coli, using three-dimensional triple-resonance techniques.
Related Articles 1H, 15N, and 13C NMR signal assignments of IIIGlc, a signal-transducing protein of Escherichia coli, using three-dimensional triple-resonance techniques.
Biochemistry. 1991 Oct 15;30(41):10043-57
Authors: Pelton JG, Torchia DA, Meadow ND, Wong CY, Roseman S
IIIGlc is an 18.1-kDa signal-transducing phosphocarrier protein of the phosphoenolpyruvate:glycose phosphotransferase...
nmrlearner
Journal club
0
08-21-2010 11:12 PM
[NMR paper] 1H, 15N, and 13C NMR signal assignments of IIIGlc, a signal-transducing protein of Es
1H, 15N, and 13C NMR signal assignments of IIIGlc, a signal-transducing protein of Escherichia coli, using three-dimensional triple-resonance techniques.
Related Articles 1H, 15N, and 13C NMR signal assignments of IIIGlc, a signal-transducing protein of Escherichia coli, using three-dimensional triple-resonance techniques.
Biochemistry. 1991 Oct 15;30(41):10043-57
Authors: Pelton JG, Torchia DA, Meadow ND, Wong CY, Roseman S
IIIGlc is an 18.1-kDa signal-transducing phosphocarrier protein of the phosphoenolpyruvate:glycose phosphotransferase...
Frequency of signal in the second dymension
Linear Mode
