[Question from NMRWiki Q&A forum] eretic standard sample
eretic standard sample
I have recently set up the eretic sample concentration measurement experiment experiment on our AvanceIII instrument. It seems to work well but the accuracy of the technique depends on the standard sample used to calibrate, I used the Bruker 48.5 mM triphenylphosphate standard since it would be good for 1H, 13C and 31P and its concentration is known fairly precisely however I have been advised that these standards from bruker use thinner walled tubes than most of my users purchase and that therefore they have a different volume and this will lead to errors to...
nmrlearner
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06-19-2012 01:38 PM
[Question from NMRWiki Q&A forum] spinning heating sample in MAS
spinning heating sample in MAS
there are two known mechanisms: friction heating( which may cause several degrees temperature increase) and induced AC current heating( in this paper:Electrical and ionic conductivity effects on magic-angle spinning nuclear magnetic resonance parameters of CuI. which can heat sample over 100 Celsius.)My question is, has any body seen this heating in MAS NMR?
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nmrlearner
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04-30-2012 05:23 AM
[Question from NMRWiki Q&A forum] 2DNOESY with N15 C13 Labelled sample or Unlabelled sample
2DNOESY with N15 C13 Labelled sample or Unlabelled sample
Dear Friends, I am in process of doing structure calculation of dimeric protein 22kD by using ARIA2.2 . Can I acquire 2DNOESY spectrum for my protein. Will it provide useful information for structure calculation? And whether I should acquire 2DNOESY with N15 C13 Labelled sample or Unlabelled sample . Which sample would be appropriate for this kind of experiment on VNMRJ 700 with biopack ?
Thanks & Regards Srinivas
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nmrlearner
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02-06-2012 03:54 PM
[Question from NMRWiki Q&A forum] sample not spining
sample not spining
HII am faceing spining problem on av-300 bruker instrument.I have change spinner,clean shim stack but the problem is still there have any body idea what i have do now,I have check by turning on and off spinner change spin rate but it not getting spinning.IS it electronic fault?
with regardferoon
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nmrlearner
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09-10-2011 08:52 PM
NMR sample.JPG
http://upload.wikimedia.org/wikimedia/commons/thumb/b/b0/NMR_sample.JPG/225px-NMR_sample.JPG
Uploaded by user "Armando-Martin" on Mon, 25 Oct 2010 01:05:00 UTC
Added to category on Mon, 25 Oct 2010 01:05:51 UTC
Original image: 1704×2272 pixel; 666.542 bytes.
Licensing : Public domain
NMR sample.JPG
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nmrlearner
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11-01-2010 08:38 AM
Sample preparation
Sample preparation Samples
The Samples program is designed to collect and store some essential information pertaining to the samples used to record the spectra.
Residual Dipolar Couplings
Bicelle preparation notes from Avanti
Instructions for bicelle preparation from Avanti