hi, why ... produces different result as ... I'm trying to develop 'ideal phase finder'. I want to apply PS as late as possible. Currently only EXT and POLY
[NMRpipe Yahoo group] Re: exchanging EXT and PS
Re: exchanging EXT and PS
Hi, I think the major reason for that is the first order phasing: it depends on the pivot point, if you apply phasing before extract it takes a specific pivot
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11-25-2012 11:59 PM
[NMRpipe Yahoo group] Re: exchanging EXT and PS
Re: exchanging EXT and PS
First order phase correction is a phase correction which changes linearly from the first point in the spectrum to the last point. A code example is below. The
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11-25-2012 11:59 PM
A 2D 13C-CEST experiment for studying slowly exchanging protein systems using methyl probes: an application to protein folding
A 2D 13C-CEST experiment for studying slowly exchanging protein systems using methyl probes: an application to protein folding
Abstract A 2D 13C Chemical Exchange Saturation Transfer (CEST) experiment is presented for studying slowly exchanging protein systems using methyl groups as probes. The utility of the method is first established through studies of protein L, a small protein, for which chemical exchange on the millisecond time-scale is not observed. Subsequently the approach is applied to a folding exchange reaction of a G48M mutant Fyn SH3 domain, for which only cross-peaks...
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06-16-2012 06:01 AM
[U. of Ottawa NMR Facility Blog] Sorting Out NOE's for Exchanging Rotomers
Sorting Out NOE's for Exchanging Rotomers
2D NOESY spectra contain cross peaks from both NOE interactions and peaks due to rotomers in slow exchange with one another on the NMR tine scale. For small molecules, the cross peaks resulting from slowly exchanging rotomers are of the same sign as the diagonal peaks. The NOE cross peaks, on the other hand, are of opposite sign compared to the diagonal peaks. When both types of correlations are present there may be more NOE correlations than expected. What follows is an example of this. The figure below shows a color coded chemical structure of a...
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01-19-2012 09:19 PM
Using the water signal to detect invisible exchanging protons in the catalytic triad of a serine protease
Using the water signal to detect invisible exchanging protons in the catalytic triad of a serine protease
Abstract Chemical Exchange Saturation Transfer (CEST) is an MRI approach that can indirectly detect exchange broadened protons that are invisible in traditional NMR spectra. We modified the CEST pulse sequence for use on high-resolution spectrometers and developed a quantitative approach for measuring exchange rates based upon CEST spectra. This new methodology was applied to the rapidly exchanging Hδ1 and Hε2 protons of His57 in the catalytic triad of bovine chymotrypsinogen-A...
exchanging EXT and PS
Linear Mode
