Question from NMRWiki Q&A forum Dimer and tetramer equilibrium identification of protein with NMR experiments

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] Dimer and tetramer equilibrium identification of protein with NMR experiments

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-22-2011, 03:51 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Dimer and tetramer equilibrium identification of protein with NMR experiments

Dimer and tetramer equilibrium identification of protein with NMR experiments

Dear nmr wikiers

could you please let me know is their any nmr experment to identification of Dimer and tetramer equilibrium of protein with NMR experiments ?

thanks in advance .

Regards

Sri

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliat The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures. Related Articles The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures. Structure. 2002 May;10(5):673-86 Authors: Barrientos LG, Louis JM, Botos I, Mori T, Han Z, O'Keefe BR, Boyd MR, Wlodawer A, Gronenborn AM The structure of the potent HIV-inactivating protein cyanovirin-N was previously found by NMR to be a monomer in solution and a domain-swapped dimer by X-ray...	nmrlearner	Journal club	0	11-24-2010 08:49 PM
[NMR tweet] Nuclear magnetic resonance parameters in water dimer http://dlvr.it/8sgrR Nuclear magnetic resonance parameters in water dimer http://dlvr.it/8sgrR Published by OrangeBezel (Orange Bezel) on 2010-11-20T13:07:05Z Source: Twitter	nmrlearner	Twitter NMR	0	11-20-2010 01:11 PM
[NMR paper] Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven b Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven by autolysis. Related Articles Real time NMR monitoring of local unfolding of HIV-1 protease tethered dimer driven by autolysis. FEBS Lett. 2001 May 18;497(1):59-64 Authors: Panchal SC, Bhavesh NS, Hosur RV Structural studies in proteases have been hampered because of their inherent autolytic function. However, since autolysis is known to be mediated via protein unfolding, careful monitoring of the autolytic reaction has the potential to throw light on the...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
[NMR paper] A conformational equilibrium in a protein fragment caused by two consecutive capping A conformational equilibrium in a protein fragment caused by two consecutive capping boxes: 1H-, 13C-NMR, and mutational analysis. Related Articles A conformational equilibrium in a protein fragment caused by two consecutive capping boxes: 1H-, 13C-NMR, and mutational analysis. Protein Sci. 1998 Jul;7(7):1506-15 Authors: Guerois R, Cordier-Ochsenbein F, Baleux F, Huynh-Dinh T, Neumann JM, Sanson A The conformational properties of an 18 residues peptide spanning the entire sequence, L1KTPA5QFDAD10ELRAA15MKG, of the first helix (A-helix) of...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] NMR solution structure of the C-terminal fragment 255-316 of thermolysin: a dimer for NMR solution structure of the C-terminal fragment 255-316 of thermolysin: a dimer formed by subunits having the native structure. Related Articles NMR solution structure of the C-terminal fragment 255-316 of thermolysin: a dimer formed by subunits having the native structure. Biochemistry. 1994 Dec 13;33(49):14834-47 Authors: Rico M, JimÃ©nez MA, GonzÃ¡lez C, De Filippis V, Fontana A The solution structure of the C-terminal fragment 255-316 of thermolysin has been determined by two-dimensional proton NMR methods. For this disulfide-free...	nmrlearner	Journal club	0	08-22-2010 03:29 AM
[NMR paper] NMR and protein folding: equilibrium and stopped-flow studies. NMR and protein folding: equilibrium and stopped-flow studies. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles NMR and protein folding: equilibrium and stopped-flow studies. Protein Sci. 1993 Dec;2(12):2007-14 Authors: Frieden C, Hoeltzli SD, Ropson IJ NMR studies are now unraveling the structure of intermediates...	nmrlearner	Journal club	0	08-22-2010 03:01 AM
[NMR paper] Dimer initiation sequence of HIV-1Lai genomic RNA: NMR solution structure of the exte Dimer initiation sequence of HIV-1Lai genomic RNA: NMR solution structure of the extended duplex. Related Articles Dimer initiation sequence of HIV-1Lai genomic RNA: NMR solution structure of the extended duplex. J Biomol Struct Dyn. 1999 Jun;16(6):1145-57 Authors: Girard F, Barbault F, Gouyette C, Huynh-Dinh T, Paoletti J, Lancelot G The genome of all retrovirus consists of two copies of genomic RNA which are noncovalently linked near their 5' end. A sequence localized immediately upstream from the splice donor site inside the HIV-1 psi-RNA...	nmrlearner	Journal club	0	08-21-2010 04:03 PM
Nitrogen-detected CAN and CON experiments as alternative experiments for main chain N Abstract Heteronuclear direct-detection experiments, which utilize the slower relaxation properties of low Î³ nuclei, such as 13C have recently been proposed for sequence-specific assignment and structural analyses of large, unstructured, and/or paramagnetic proteins. Here we present two novel 15N direct-detection experiments. The CAN experiment sequentially connects amide 15N resonances using 13CÎ± chemical shift matching, and the CON experiment connects the preceding 13Câ?² nuclei. When starting from the same carbon polarization, the intensities of nitrogen signals detected in the CAN or...	nmrlearner	Journal club	0	08-14-2010 04:19 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off