[Question from NMRWiki Q&A forum] Data processing and Sparky

[nmrlearner]

Data processing and Sparky

Dear NMRWikiers, I'm fresh in nmr field and would like to ask couple questions: 1. Our protein has about 132 residues.The NHSQC is well dispersed but many peaks can't be found in CBCANH and CBCACONH. HNCA/HNCOCA + HNCO/HNCACO are planned for backbone assignment. Other people processed data with NMRPipe. The NHSQC peaks can mostly find matching peaks in HNCO/HNCACO, while HNCA/HNCOCA can't be matched (no same resonances can be seen when synchronized). But, many peaks can be seen in HNCA/HNCOCA. I found that the whole scale of 13C axis in HNCA is from 51 to 66 and the one in HNCOCA is from 42 to 73. Maybe this is the reason for peak un-matching in these two spectra. Where is the problem in processing? Is it possible to correct by re-processing? 2. I'm using SPARKY to do assignment. In Strip Plot, after "Add selected strip peaks" (HNCO + HNCACO),Pointer Mode in "find/add peak", pick the peak in HNCACO, hold "Shift" down and click HNCO window and selected, go to "Find - Add strips matching peaks", but only HNCACO strips are added, never HNCO. What's wrong?

Many thanks in advance,

John Leehono



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