I am running COSY on a DNA:RNA hybrifd. However, I have got no cross-peaks at all even for CYT H5-H6? what do you think the problem is? I am using cosydfesgpph as pulse programme.
Thank you
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[Question from NMRWiki Q&A forum] COSY problem (cosydfesgpph pulse program)
COSY problem (cosydfesgpph pulse program)
Hi,
I am running COSY on a DNA:RNA hybrifd. However, I have got no cross-peaks at all even for CYT H5-H6? what do you think the problem is? I am using cosydfesgpph as pulse programme.
Thank you
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Spin-spin coupling constants can have values greater than or less than zero. The absolute sign of the coupling constants cannot be discerned from the simple examination of a 1H NMR spectrum. The E.COSY1 (Exclusive COrrelation SpectroscopY) technique is one method which can be used to determine the relative signs of coupling constants. E.COSY is a phase sensitive COSY variant which produces off-diagonal signals showing only the active coupling (i.e. the coupling directly responsible for the cross-peak) as 2x2 antiphase square tetrads...
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