Hello all, In some interleaved relaxation experiments, we've noticed that one of our spectrometers is having trouble locking and the signals (FIDs) shift ever
[NMRpipe Yahoo group] Correct for a shifting lock
Correct for a shifting lock
Hello all, In some interleaved relaxation experiments, we've noticed that one of our spectrometers is having trouble locking and the signals (FIDs) shift ever
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01-13-2012 11:04 PM
[Question from NMRWiki Q&A forum] NMR Lock signal
NMR Lock signal
THis is my lock signal....what is up with it, and what do I do?https://picasaweb.google.com/113100759436397078363/LockSignal02#slideshow/5631842657786659330
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nmrlearner
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07-21-2011 10:31 PM
[Question from NMRWiki Q&A forum] Can correct TAUc be a sufficient evidence of the absence of aggregation?
Can correct TAUc be a sufficient evidence of the absence of aggregation?
Hello!
I am studying the protein backbone dynamics using the standard set of NMR relaxation parameters (R1, R2, NOE). I would like to be sure that there is no aggregation in the sample, and I have doubts about it so far. The best evidence I have is that overall rotational correlation time TAUc, predicted by HYDRONMR, matches exactly the one calculated from averaged R2/R1 values. Can anyone tell me for sure it is enough and I can be sure that protein doesn't form dimers/oligomers?
Thanks!
nmrlearner
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06-10-2011 01:50 PM
[Question from NMRWiki Q&A forum] NMR resonance Lock
NMR resonance Lock
I am running a varian gemini 300 on SUN microsystems with UNIX. I am trying to lock on my sample, and my lock is not showing up as a "step" as usual. Instead, it has troughs in it. What are some options in fixing this, or is this acceptable?
-Jon K.
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nmrlearner
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06-07-2011 01:01 AM
[Question from NMRWiki Q&A forum] Correct mathematical formulas of the important window functions
Correct mathematical formulas of the important window functions
Hi,
regarding my C++/Qt NMR processing application (see http://qa.nmrwiki.org/question/149/problem-reading-and-transforming-vendor-data-using-cqt or http://cutenmr.sourceforge.net/) I have a question concerning the window functions.
I did research in the literature but could not find the "correct" mathematical formulas of the important window functions (exponential, gaussian, sine, sine squared, TRAF). For the gaussian function I found:
exp(-(t*LB)^2)
nmrlearner
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09-19-2010 06:03 PM
[NMR software blog] Can Zero-Filling Correct the Baseline?
Can Zero-Filling Correct the Baseline?
I want to show you a proton spectrum that has puzzled me during the last weeks. It contains something that's quite typical and something that I can't explain. I have processed the spectrum in two different ways, with zero-filling and without it. The spectrum without zero-filling is black, the spectrum with zero-filling is green (the number of points is doubled).
http://2.bp.blogspot.com/_ROkYShLRKqA/TGu61b5bG7I/AAAAAAAAAUQ/9AbsXlWbKUY/s400/TMS.pngThis detail is the bottom part of the TMS signal (magnified to show the ringing effect). Where does the...
nmrlearner
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08-21-2010 06:29 PM
Correct mis-referenced NMR shifts with MANI-LACS
MANI-LACS Server v. 0.1 (Beta)
MANI-LACS offers a new approach to NMR reference correction and outlier identification that achieves excellent results using only residue specific chemical shift assignment.
http://bija.nmrfam.wisc.edu/MANI-LACS/seeds.jpg
Instructions for preparing input files
Database of chemical shift corrections
nmrlearner
General
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08-14-2005 09:11 PM
New way to correct misreferenced chemical shifts
A simple method to adjust inconsistently referenced 13C and 15N chemical shift assignments of proteins.
Wang Y, Wishart DS.
J Biomol NMR. 2005 Feb;31(2):143-8.
http://pronmr.com/yunjunwang_files/yjw_pssi.jpg
Abstract:
Correct for a shifting lock
Linear Mode
