Question from NMRWiki Q&A forum Can correct TAUc be a sufficient evidence of the absence of aggregation?

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[Question from NMRWiki Q&A forum] Can correct TAUc be a sufficient evidence of the absence of aggregation?

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06-10-2011, 01:50 PM

nmrlearner

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Can correct TAUc be a sufficient evidence of the absence of aggregation?

Can correct TAUc be a sufficient evidence of the absence of aggregation?

Hello!

I am studying the protein backbone dynamics using the standard set of NMR relaxation parameters (R1, R2, NOE). I would like to be sure that there is no aggregation in the sample, and I have doubts about it so far. The best evidence I have is that overall rotational correlation time TAUc, predicted by HYDRONMR, matches exactly the one calculated from averaged R2/R1 values. Can anyone tell me for sure it is enough and I can be sure that protein doesn't form dimers/oligomers?

Thanks!

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