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Old 06-29-2012, 08:44 AM
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Default A beginner's guide to protein backbone assignment?

A beginner's guide to protein backbone assignment?

Hi All,

I'm new to protein backbone assignment by NMR, and my protein is about ~37kDa (about 400 residues). We've acquired a series of triple resonance spectra: HNCA, HNCACB, HNCOCACB, HNCOCA, and HNCACO. I also have an HQCS spectrum. While I know how magnetization is transferred in the above mentioned experiment, how do I start assigning my protein? I use NMRpipe to process the spectra and Sparky for assignment.

Thanks a lot!



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