Question from NMRWiki Q&A forum Anti-diagonal artefact in DQF COSY (Varian dqcosy.c)

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[Question from NMRWiki Q&A forum] Anti-diagonal artefact in DQF COSY (Varian dqcosy.c)

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NMR processing:

MDD

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Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

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GeNMR

I-TASSER

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Amber

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Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

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RCI

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HADDOCK

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MestReS

V-NMR

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Methyl S2

B-factor

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Amber

Antechamber

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Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

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DisMeta

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08-06-2015, 10:24 AM

nmrlearner

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Anti-diagonal artefact in DQF COSY (Varian dqcosy.c)

Anti-diagonal artefact in DQF COSY (Varian dqcosy.c)

Hi I have the following problem in dqfcosy - have pretty strong antidiagonal.

I've run the same experiment before on other samples and did not have this problem, could you suggest anything? Is there any other pulse sequence in the Varian toolchest superior to this one?

... but probably something is just wrong with my setup, it is a really simple sequence though and I thought it would be hard to screw up :). Or is it normal and I just have low S/N?

edit: I've figured out that in this case I had receiver overflow. To test that go to the .fid directory of the experiment and inspect file called log.

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