Question from NMRWiki Q&A forum 3dnmr CBCANH experment

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[Question from NMRWiki Q&A forum] 3dnmr CBCANH experment

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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05-12-2011, 07:41 PM

nmrlearner

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3dnmr CBCANH experment

3dnmr CBCANH experment

Dear NMR wiki ers

Iam working on protein family which can form dimers (total 20kd size ) using 25mM TRIS , 100 mM KCL Ph-6.8 , protein conc -2mM ( limitaion )

Iam always geting bad quality CBCANH experment on 700 vnmr cryob Could you please suggest me about expermental parameters or buffer conditions to get good quality of CBCANH experment

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CBCANH experment on 700 vnmr Iam working on protein family which can form dimers (total 20kd size ) using 25mM TRIS , 100 mM KCL Ph-6.8 , protein conc -2mM ( limitaion ) Iam always geting bad quality of CBCANH contures experment on 700 vnmr cryob . quality of N-H hsqc is good Could you please suggest me about expermental parameters or buffer conditions to get good quality of CBCANH experment	penumutchu.srinivas	NMR Questions and Answers	2	11-15-2011 11:46 AM
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[NMRwiki tweet] nmrwiki: How to improve quality of CBCANH #nmr data? http://qa.nmrwiki.org/question/258/3dnmr-cbcanh-experment nmrwiki: How to improve quality of CBCANH #nmr data? http://qa.nmrwiki.org/question/258/3dnmr-cbcanh-experment nmrwiki: How to improve quality of CBCANH #nmr data? http://qa.nmrwiki.org/question/258/3dnmr-cbcanh-experment Source: NMRWiki tweets	nmrlearner	Twitter NMR	0	05-13-2011 07:41 AM
[NMR paper] Sequential HNCACB and CBCANH protein NMR pulse sequences. Sequential HNCACB and CBCANH protein NMR pulse sequences. Related Articles Sequential HNCACB and CBCANH protein NMR pulse sequences. J Magn Reson. 2001 Aug;151(2):328-31 Authors: Meissner A, Sørensen OW The pulse sequences HNCACB and CBCANH correlating side chain C(beta) resonances with amide resonances in the protein backbone do not distinguish between inter- and intraresidue correlations. The new pulse sequences sequential HNCACB and sequential CBCANH make this distinction by suppressing coherence transfer between 13C(alpha) and 15N via the...	nmrlearner	Journal club	0	11-19-2010 08:44 PM

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