Question from NMRWiki Q&A forum 3d Tocsy-hsqc mgnetization transfer problem

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[Question from NMRWiki Q&A forum] 3d Tocsy-hsqc mgnetization transfer problem

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NMR processing:

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NOEs:

Structure from NMR restraints:

Ab initio:

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Rosetta-NMR (Robetta)

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GeNMR

I-TASSER

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Amber

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Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

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CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

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RCI

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HADDOCK

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Methyl S2

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Shiftx2

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CH3shift- Methyl

ArShift- Aromatic

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CheShift-2- Cα

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Shifty

Camcoil

Poulsen_rc_CS

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MAXOCC

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CCPN

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Solid-state NMR:

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03-06-2012, 06:17 PM

nmrlearner

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3d Tocsy-hsqc mgnetization transfer problem

3d Tocsy-hsqc mgnetization transfer problem

Dear All,I acquired a 3d tocsy-hsqc edited on Bruker machine of small protein in micelles with a mixing time of 70ms. The problem is that I do not observe almoust spin system of each aminoacid, in detail I see only cross peaks between NH-HA. I obtained very good results by 3d Noesy-hsqc edited. How can I do to improve the magnetization transfer along the spin system and see all cross peaks? Do you think that this problem is due to the nature?Thank you in advance\

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