Question from NMRWiki Q&A forum 2DNOESY with N15 C13 Labelled sample or Unlabelled sample

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] 2DNOESY with N15 C13 Labelled sample or Unlabelled sample

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-06-2012, 03:54 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

2DNOESY with N15 C13 Labelled sample or Unlabelled sample

2DNOESY with N15 C13 Labelled sample or Unlabelled sample

Dear Friends, I am in process of doing structure calculation of dimeric protein 22kD by using ARIA2.2 . Can I acquire 2DNOESY spectrum for my protein. Will it provide useful information for structure calculation? And whether I should acquire 2DNOESY with N15 C13 Labelled sample or Unlabelled sample . Which sample would be appropriate for this kind of experiment on VNMRJ 700 with biopack ?

Thanks & Regards Srinivas

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] sample not spining sample not spining HII am faceing spining problem on av-300 bruker instrument.I have change spinner,clean shim stack but the problem is still there have any body idea what i have do now,I have check by turning on and off spinner change spin rate but it not getting spinning.IS it electronic fault? with regardferoon Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	09-10-2011 08:52 PM
[U. of Ottawa NMR Facility Blog] Sample Slice Selection in NMR Spectroscopy Sample Slice Selection in NMR Spectroscopy In MRI, field gradients are used routinely for slice selection while imaging a sample. Gradients are also finding applications in high resolution NMR spectroscopy employing fast data collection techniques using parallel data acquisition for multiple slices of the same sample. A good example of this can be found in Carlos' BLOG (and the references therein). The slice selection is accomplished by turning on a linear field gradient across the sample while applying an excitation pulse. While the gradient is on, the frequencies of each of the NMR...	nmrlearner	News from NMR blogs	0	01-07-2011 12:01 AM
NMR sample.JPG http://upload.wikimedia.org/wikimedia/commons/thumb/b/b0/NMR_sample.JPG/225px-NMR_sample.JPG Uploaded by user "Armando-Martin" on Mon, 25 Oct 2010 01:05:00 UTC Added to category on Mon, 25 Oct 2010 01:05:51 UTC Original image: 1704×2272 pixel; 666.542 bytes. Licensing : Public domain NMR sample.JPG More...	nmrlearner	NMR pictures	0	11-01-2010 08:38 AM
[Question from NMRWiki Q&A forum] How many peaks should be in DOSY spectrum of D2O sample? How many peaks should be in DOSY spectrum of D2O sample? Hello, I've recorded a DOSY data set and processed with Bayesian DOSY transform. There seem to be at least two peaks in the processed spectrum. Is the second one an artifact? In theory there might be a signal of H2O along with HDO, but wouldn't exchange of deuterium be much faster than the experiment time scale? Thanks.	nmrlearner	News from other NMR forums	0	08-28-2010 10:31 AM
NMR Sample Preparation from NESG Wiki NMR Sample Preparation from NMR2.0 Wiki http://www.nmr2.buffalo.edu/nesg.wiki/NMR_Sample_Preparation originally posted by Main.Gaohua.Liu (11 Feb 2007) <!-- start content --> <table style="width: 76px; height: 63px;" id="toc" class="toc" summary="Contents"><tbody><tr><td>Contents	markber	BioNMR Wiki	0	08-16-2010 04:04 AM
Sample preparation Sample preparation Samples The Samples program is designed to collect and store some essential information pertaining to the samples used to record the spectra. Residual Dipolar Couplings Bicelle preparation notes from Avanti Instructions for bicelle preparation from Avanti	ale51	BioNMR Wiki	0	01-29-2005 01:35 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off