Question from NMRWiki Q&A forum 2D ROESY-NMR with ~0 peak intensity

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[Question from NMRWiki Q&A forum] 2D ROESY-NMR with ~0 peak intensity

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

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NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

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SAVES2 or SAVES4

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FANDAS

MestReS

V-NMR

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Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

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Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

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NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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01-18-2014, 11:31 AM

nmrlearner

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2D ROESY-NMR with ~0 peak intensity

2D ROESY-NMR with ~0 peak intensity

I have been trying to elucidate the 3D structure of oxidized glutathione (M.Wt= 612.63 g/mol) with the help of roesy distance restrains. However, the intensity of roesy peaks is coming out to be 0.Instrument: Bruker Avance II (2005 model) spectrometer. operating frequency: 400 MHz Molar concentration of GSSG= 200mM, pH=7.4Mixing time= 600msecRelaxation delay= 3 Secprocessor= ACD/NMR processorPlease give some suggestions to avail appropriate peak intensity.

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