Question from NMRWiki Q&A forum 2D Filtered NOESY/TOCSY on Varian Biopack

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] 2D Filtered NOESY/TOCSY on Varian Biopack

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

12-20-2010, 01:52 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,780

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

2D Filtered NOESY/TOCSY on Varian Biopack

2D Filtered NOESY/TOCSY on Varian Biopack

I am attempting to run some filtered NOESY and TOCSY experiments using sequences in the Varian Biopack - CNfiltocsy and CNfilnoesy. The experiments seem to run fine, but when overlaying the processed spectra something seems odd - the TOCSY crosspeaks are not present in the NOESY spectra and vice-versa. Does anyone know why this might be the case?

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] Isotope Filtered NOESY Isotope Filtered NOESY I'm an NMR newbie. I know isotope filtering can be used to study a complex under slow exchange, but for fast-exchange, is it possible to acquire only the intramolecular NOEs of the receptor as opposed to intermolecular NOEs between the receptor and ligand? I just want the contacts in the receptor. Thanks. Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	02-06-2012 03:50 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY Re: N15 separated NOESY and TOCSY Thank you for your replies. The processing script of TOCSY (NH plane): $NMRTXT/ext.xz.com fid/test%03d.fid xz001.fid nmrPipe -in xz001.fid \ ... -out xzf.ft2 More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-29-2011 11:00 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY Re: N15 separated NOESY and TOCSY Hi Iris, Another question is what do you mean by the NH vs HH planes in regards to the 3D matrix (a single 3D peak can't be positive viewed along one axis and More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-27-2011 10:40 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY Re: N15 separated NOESY and TOCSY Hi Iris, It's difficult to diagnose the problem without seeing the processing script, but what phase correction was used in the 15N dimension? If the initial More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-26-2011 10:34 PM
[NMRpipe Yahoo group] N15 separated NOESY and TOCSY N15 separated NOESY and TOCSY Hello, I did a N15 separated TOCSY experiment and used the following script: #!/bin/csh bruk2pipe -in ./ser \ -bad 0.0 -aswap -DMX -decim 2632 -dspfvs 20 More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-26-2011 10:34 PM
[NMRwiki tweet] nmrwiki: How does filtered NOE work? #nmrhttp://qa.nmrwiki.org/question/235/basics-of-filtered-noe-experiment nmrwiki: How does filtered NOE work? #nmrhttp://qa.nmrwiki.org/question/235/basics-of-filtered-noe-experiment nmrwiki: How does filtered NOE work? #nmrhttp://qa.nmrwiki.org/question/235/basics-of-filtered-noe-experiment Source: NMRWiki tweets	nmrlearner	Twitter NMR	0	03-04-2011 02:12 AM
[Question from NMRWiki Q&A forum] Varian Biopack pulse sequences for intermolecular NOEs Varian Biopack pulse sequences for intermolecular NOEs Hi All, I'm trying to set up some experiments to determine intermolecular NOEs in a peptide:protein complex. At the moment from looking at the Biopack library, I have found 4 pulse sequences which will do this and each provide different information (gnoesyNhsqc_CN, gnoesyNhsqc_NN, gnoesyChsqc_CC and gnoesyChsqc_NC). Would people recommend using a combination of all four of these experiments to determine the intermolecular NOEs for use in structure calculations? Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	08-22-2010 02:30 AM
[NMRwiki tweet] nmrwiki: know #varian biopack pulse sequences for intermolecular noes? #nmr #chemistr nmrwiki: know #varian biopack pulse sequences for intermolecular noes? #nmr #chemistry http://qa.nmrwiki.org/question/154/ nmrwiki: know #varian biopack pulse sequences for intermolecular noes? #nmr #chemistry http://qa.nmrwiki.org/question/154/ Source: NMRWiki tweets	nmrlearner	Twitter NMR	0	08-22-2010 01:49 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off