Question from NMRWiki Q&A forum 2D 103Rh frequency wrong

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] 2D 103Rh frequency wrong

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-01-2013, 12:26 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,778

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

2D 103Rh frequency wrong

2D 103Rh frequency wrong

I have run a 1H-103Rh HMQC on [Rh(acac)(cod)] and found the chemical shift of the 103Rh to be -7023 ppm. The frequency of the 103Rh was first obtained by running a paropt on the "decp90" pulse program and changing O2 in order to decouple the 103Rh. When I found the frequency at which the 103Rh decouple, I used that value for O2 in the 2D 1H-103Rh HMQC. However, I am sure that this value of -7203 ppm is incorrect, since all the literature references to 103Rh NMR states that the chemical shift range for 103Rh is between -2000 and 10000 ppm. Also, [Rh(acac)(cod)] is given in literature as having a chemical shift of +1287 ppm.

I know that the peak is not folded, since I acquired a spectrum with the exactly the same parameters, but with O2p at 1000 ppm, but then I don't see any 1H-103Rh correlation. I am obviously doing something wrong, but can't figure out what. Have anyone encountered this with any X nuclei? The data were acquired on our 600 MHz Bruker Avance II, using a 5 mm TBI 1H/31P/BB inverse probe.

Any advice on the matter will be appreciated.

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] what went wrong? what went wrong? a query again? I recorded an DQF on deuterated solid sample. The basic pulse programme taken from bruker, optimised the pulse and kept the proper short delay between the last two 90 pulses? phase cycling is same as mentioned in bruker? I got empty spectrum with very poor diagonal and no cross peaks? what could possibly go wrong ? does these delays depend on higher magnetic field strengths? or pulse imperfections cause collapsed spectrum or empty spectrum? the spectra i recorded on 700 MHz with 14 KHz MAS.	nmrlearner	News from other NMR forums	0	11-18-2011 06:51 PM
[Question from NMRWiki Q&A forum] incorrect processing of 2D HSQC in NMRPipe - what is wrong? incorrect processing of 2D HSQC in NMRPipe - what is wrong? i'm trying to process an HSQC by using standard scripts of NMRpipe, and get this: <img src="http://qa.nmrwiki.org/upfiles/13022976745696182.png" width="50%" /img> what is wrong in my processing? at the spectrometer the spectrum looked nice, so it's a matter of processing... conversion:	nmrlearner	News from other NMR forums	0	04-09-2011 12:17 AM
[Question from NMRWiki Q&A forum] What's wrong with this spectrum? Bruker TPPI 2D TOCSY What's wrong with this spectrum? Bruker TPPI 2D TOCSY Hi, I think this is probably a basic problem but I'm a bit inexperienced with NMR. I'm just trying to process an ethanol TOCSY spectrum from the biomolecular magnetic resonance data bank, but strange peaks are appearing at the very edge of the data. The spectrum should look like this (processed in SpinWorks): <img src="http://qa.nmrwiki.org/upfiles/12889441489353594.png" width="70%"</img>	nmrlearner	News from other NMR forums	0	11-05-2010 12:20 PM
[Stan NMR blog] Are Bruker and Jeol NMR data wrong? Are Bruker and Jeol NMR data wrong? Recent FID's acquired with Bruker and Jeol spectrometers exhibit an anomalous group delay artifact ... More...	nmrlearner	News from NMR blogs	0	08-21-2010 05:42 PM
why radio frequency Dear sir, Why we are using radio frequency excluding other electro magnetic radiation. thankx	nbkoneru	NMR Questions and Answers	1	07-02-2006 06:50 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off