Hello All, I am evaluation the 15N line width of my 1H-15N HSQC spectrum. From the peak list I found the average 15N line width is ~9 Hz. And I found from the
[Question from NMRWiki Q&A forum] Line broadening in nmr
Line broadening in nmr
Hello can some body help me in understanding the line broadening effect in NMR? I am working with protein and small molecules and I observed severe broadening at a protein to ligand ratio 1:20. I am pretty sure that its not aggregation since i have the free ligand spectrum without the protein which didnt showed any broadening effect.
Hope somebody will guide me..
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10-04-2011 08:47 PM
[NMRpipe Yahoo group] Re: 15N line width question
Re: 15N line width question
Wenli, three things: (1) the linewidth is proportional with the tumbling time of your protein, so one can't generalize (2) it refers to the natural linewidth,
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01-19-2011 02:50 AM
[NMR paper] Line shape considerations in ultrafast 2D NMR.
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J Magn Reson. 2004 Feb;166(2):152-63
Authors: Shapira B, Lupulescu A, Shrot Y, Frydman L
We have recently proposed and demonstrated an approach that enables the acquisition of 2D nuclear magnetic resonance (NMR) spectra within a single scan. The approach is based on spatially encoding the spins' evolution along the indirect domain with the aid of a magnetic field gradient, and subsequently decoding this information numerous times over...
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11-24-2010 09:25 PM
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Major groove width variations in RNA structures determined by NMR and impact of 13C r
Abstract Ribonucleic acid structure determination by NMR spectroscopy relies primarily on local structural restraints provided by 1Hâ?? 1H NOEs and J-couplings. When employed loosely, these restraints are broadly compatible with A- and B-like helical geometries and give rise to calculated structures that are highly sensitive to the force fields employed during refinement. A survey of recently reported NMR structures reveals significant variations in helical parameters, particularly the major groove width. Although helical parameters observed in high-resolution X-ray crystal structures of...
15N line width question
Linear Mode
