Towards structure determination of self-assembled peptides using dynamic nuclear polarization enhanced solid-state NMR spectroscopy

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Towards structure determination of self-assembled peptides using dynamic nuclear polarization enhanced solid-state NMR spectroscopy

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NMR processing:

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Torsion angles from chemical shifts:

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Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

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04-23-2014, 06:31 PM

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Towards structure determination of self-assembled peptides using dynamic nuclear polarization enhanced solid-state NMR spectroscopy

From The DNP-NMR Blog:

Towards structure determination of self-assembled peptides using dynamic nuclear polarization enhanced solid-state NMR spectroscopy

Takahashi, H., et al., Towards structure determination of self-assembled peptides using dynamic nuclear polarization enhanced solid-state NMR spectroscopy. Angew Chem Int Ed Engl, 2013. 52(27): p. 6979-82.

http://www.ncbi.nlm.nih.gov/pubmed/23564735

Bio-inspired self-assemblies made of peptide building blocks have great potential for nanotechnology ranging from biological and pharmaceutical applications to (opto)electronics. With these goals, a variety of peptide nanoassemblies have been studied and designed over the last few decades. Inevitably, structural studies at an atomic scale are crucial to unravel the mechanisms that drive nanoassembly formation as well as to relate these structures to their physical and chemical properties. However, structure determination at an atomic level is challenging essentially because of the difficulty associated with using X-ray crystallography on such nanoassemblies.

Go to The DNP-NMR Blog for more info.

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