Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization

		BioNMR > NMR community > News from NMR blogs
Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-03-2015, 11:04 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization

From The DNP-NMR Blog:

Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization

Miclet E, Abergel D, Bornet A, Milani J, Jannin S, Bodenhausen G. Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization. The Journal of Physical Chemistry Letters. 2014;5(19):3290-5.

http://dx.doi.org/10.1021/jz501411d

Dissolution dynamic nuclear polarization (D-DNP) experiments enabled us to study the kinetics of the enzymatic phosphorylation reaction of glucose to form glucose-6-phosphate (G6P) by hexokinase (HK), with or without the presence of an excess of G6P, which is known to be an inhibitor of the enzyme. Against all expectations, our observations demonstrate that the phosphorylation of both ? and ? glucose anomers occurs with comparable kinetics. The catalytic constant of the reaction was estimated based on a simple kinetic model tailored for hyperpolarized systems.

Go to The DNP-NMR Blog for more info.

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization From The DNP-NMR Blog: Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization Gajan, D., et al., Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization. Proc. Nat. Aca. Sci. USA, 2014. 111(41): p. 14693-14697. http://www.pnas.org/content/111/41/14693.abstract	nmrlearner	News from NMR blogs	0	11-12-2014 03:02 PM
Cross-polarization for dissolution dynamic nuclear polarization From The DNP-NMR Blog: Cross-polarization for dissolution dynamic nuclear polarization Batel, M., et al., Cross-polarization for dissolution dynamic nuclear polarization. Phys Chem Chem Phys, 2014. 16(39): p. 21407-16. http://www.ncbi.nlm.nih.gov/pubmed/25182534	nmrlearner	News from NMR blogs	0	10-08-2014 05:52 PM
Cross Polarization for Dissolution Dynamic Nuclear Polarization From The DNP-NMR Blog: Cross Polarization for Dissolution Dynamic Nuclear Polarization Batel, M., et al., Cross Polarization for Dissolution Dynamic Nuclear Polarization. Phys. Chem. Chem. Phys., 2014. http://dx.doi.org/10.1039/C4CP02696A	nmrlearner	News from NMR blogs	0	08-22-2014 07:27 PM
Protein folding studied by dissolution dynamic nuclear polarization From The DNP-NMR Blog: Protein folding studied by dissolution dynamic nuclear polarization Chen, H.Y., M. Ragavan, and C. Hilty, Protein folding studied by dissolution dynamic nuclear polarization. Angew Chem Int Ed Engl, 2013. 52(35): p. 9192-5. http://www.ncbi.nlm.nih.gov/pubmed/23857756	nmrlearner	News from NMR blogs	0	04-07-2014 06:59 PM
Hyperpolarization of deuterated metabolites via remote cross-polarization and dissolution dynamic nuclear polarization From The DNP-NMR Blog: Hyperpolarization of deuterated metabolites via remote cross-polarization and dissolution dynamic nuclear polarization Vuichoud, B., et al., Hyperpolarization of deuterated metabolites via remote cross-polarization and dissolution dynamic nuclear polarization. J Phys Chem B, 2014. 118(5): p. 1411-5. http://www.ncbi.nlm.nih.gov/pubmed/24397585	nmrlearner	News from NMR blogs	0	04-02-2014 11:54 PM
Formulation and utilization of choline based samples for dissolution dynamic nuclear polarization From The DNP-NMR Blog: Formulation and utilization of choline based samples for dissolution dynamic nuclear polarization Bowen, S. and J.H. Ardenkjaer-Larsen, Formulation and utilization of choline based samples for dissolution dynamic nuclear polarization. J Magn Reson, 2013. 236(0): p. 26-30. http://www.ncbi.nlm.nih.gov/pubmed/24036470	nmrlearner	News from NMR blogs	0	12-02-2013 11:48 PM
Over 35% liquid-state (13)C polarization obtained via dissolution dynamic nuclear polarization at 7 T and 1 K using ubiquitous nitroxyl radicals From The DNP-NMR Blog: Over 35% liquid-state (13)C polarization obtained via dissolution dynamic nuclear polarization at 7 T and 1 K using ubiquitous nitroxyl radicals Cheng, T., et al., Over 35% liquid-state (13)C polarization obtained via dissolution dynamic nuclear polarization at 7 T and 1 K using ubiquitous nitroxyl radicals. Phys Chem Chem Phys, 2013. 15(48): p. 20819-22. http://www.ncbi.nlm.nih.gov/pubmed/24217111	nmrlearner	News from NMR blogs	0	11-29-2013 09:58 PM
Cross Polarization for Dissolution Dynamic Nuclear Polarization Experiments at Readily Accessible Temperatures 1.2* From The DNP-NMR Blog: Cross Polarization for Dissolution Dynamic Nuclear Polarization Experiments at Readily Accessible Temperatures 1.2*	nmrlearner	News from NMR blogs	0	11-21-2013 01:14 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off