SABRE is a nuclear spin hyperpolarization technique based on the reversible association of a substrate molecule and para-hydrogen (p-H2) to a metal complex. During the lifetime of such a complex, generally fractions of a second, the spin order of p-H2 is transferred to the nuclear spins of the substrate molecule via a transient scalar coupling network, resulting in strongly enhanced NMR signals. This technique is generally applied at relatively high concentrations (mM), in large excess of substrate with respect to metal complex. Dilution of substrate ligands below stoichiometry results in progressive decrease of signal enhancement, which precludes the direct application of SABRE to the NMR analysis of low concentration (muM) solutions. Here, we show that the efficiency of SABRE at low substrate concentrations can be restored by addition of a suitable coordinating ligand to the solution. The proposed method allowed NMR detection below 1 muM in a single scan.
Level Anti-Crossings are a Key Factor for Understanding para-Hydrogen-Induced Hyperpolarization in SABRE Experiments
From The DNP-NMR Blog:
Level Anti-Crossings are a Key Factor for Understanding para-Hydrogen-Induced Hyperpolarization in SABRE Experiments
Pravdivtsev, A.N., et al., Level Anti-Crossings are a Key Factor for Understanding para-Hydrogen-Induced Hyperpolarization in SABRE Experiments. ChemPhysChem, 2013. 14(14): p. 3327-3331.
http://www.ncbi.nlm.nih.gov/pubmed/23959909
The Feasibilityof Formation and Kinetics of NMR SignalAmplification by Reversible Exchange (SABRE) at High Magnetic Field(9.4 T)
The Feasibilityof Formation and Kinetics of NMR SignalAmplification by Reversible Exchange (SABRE) at High Magnetic Field(9.4 T)
Danila A. Barskiy, Kirill V. Kovtunov, Igor V. Koptyug, Ping He, Kirsten A. Groome, Quinn A. Best, Fan Shi, Boyd M. Goodson, Roman V. Shchepin, Aaron M. Coffey, Kevin W. Waddell and Eduard Y. Chekmenev
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja501052p/aop/images/medium/ja-2014-01052p_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja501052p...
nmrlearner
Journal club
0
02-25-2014 12:44 AM
TowardNanomolar Detection by NMR Through SABRE Hyperpolarization
TowardNanomolar Detection by NMR Through SABRE Hyperpolarization
Nan Eshuis, Niels Hermkens, Bram J. A. van Weerdenburg, Martin C. Feiters, Floris P. J. T. Rutjes, Sybren S. Wijmenga and Marco Tessari
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja412994k/aop/images/medium/ja-2013-12994k_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/ja412994k
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/CRRo27dOPlc
nmrlearner
Journal club
0
02-05-2014 06:08 PM
Optimization of SABRE for polarization of the tuberculosis drugs pyrazinamide and isoniazid
From The DNP-NMR Blog:
Optimization of SABRE for polarization of the tuberculosis drugs pyrazinamide and isoniazid
Zeng, H., et al., Optimization of SABRE for polarization of the tuberculosis drugs pyrazinamide and isoniazid. J Magn Reson, 2013. 237(0): p. 73-8.
http://www.ncbi.nlm.nih.gov/pubmed/24140625
nmrlearner
News from NMR blogs
0
12-18-2013 04:00 PM
Hyperpolarization Methods and Applications in NMR
From The DNP-NMR Blog:
Hyperpolarization Methods and Applications in NMR
Köckenberger, W. and J. Matysik, Hyperpolarization Methods and Applications in NMR, in Encyclopedia of Spectroscopy and Spectrometry (Second Edition), L. Editor-in-Chief: John, Editor. 2010, Academic Press: Oxford. p. 963-970.
http://dx.doi.org/10.1016/B978-0-12-374413-5.00054-3
nmrlearner
News from NMR blogs
0
08-21-2013 08:49 PM
Utilization of SABRE-DerivedHyperpolarization ToDetect Low-Concentration Analytes via 1D and 2D NMR Methods
Utilization of SABRE-DerivedHyperpolarization ToDetect Low-Concentration Analytes via 1D and 2D NMR Methods
Lyrelle S. Lloyd, Ralph W. Adams, Michael Bernstein, Steven Coombes, Simon B. Duckett, Gary G. R. Green, Richard. J. Lewis, Ryan E. Mewis and Christopher J. Sleigh
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja3051052/aop/images/medium/ja-2012-051052_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/ja3051052
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...