Topical Developments in High-Field Dynamic Nuclear Polarization

		BioNMR > NMR community > News from NMR blogs
Topical Developments in High-Field Dynamic Nuclear Polarization

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-05-2014, 01:35 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Topical Developments in High-Field Dynamic Nuclear Polarization

From The DNP-NMR Blog:

Topical Developments in High-Field Dynamic Nuclear Polarization

Michaelis, V.K., et al., Topical Developments in High-Field Dynamic Nuclear Polarization. Israel Journal of Chemistry, 2014. 54(1-2): p. 207-221.

http://dx.doi.org/10.1002/ijch.201300126

We report our recent efforts directed at improving high-field dynamic nuclear polarization (DNP) experiments. We investigated a series of thiourea nitroxide radicals and the associated DNP enhancements ranging from ?=25 to 82, which demonstrate the impact of molecular structure on performance. We directly polarized low-gamma nuclei, including 13C, 2H, and 17O, by the cross effect mechanism using trityl radicals as a polarization agent. We discuss a variety of sample preparation techniques for DNP with emphasis on the benefits of methods that do not use a glass-forming cryoprotecting matrix. Lastly, we describe a corrugated waveguide for use in a 700 MHz/460 GHz DNP system that improves microwave delivery and increases enhancements up to 50 %.

Go to The DNP-NMR Blog for more info.

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Dynamic Nuclear Polarization NMR Spectroscopy Allows High-Throughput Characterization of Microporous Organic Polymers From The DNP-NMR Blog: Dynamic Nuclear Polarization NMR Spectroscopy Allows High-Throughput Characterization of Microporous Organic Polymers Blanc, F., et al., Dynamic nuclear polarization NMR spectroscopy allows high-throughput characterization of microporous organic polymers. J Am Chem Soc, 2013. 135(41): p. 15290-3. http://www.ncbi.nlm.nih.gov/pubmed/24028380	nmrlearner	News from NMR blogs	0	12-11-2013 08:50 PM
Temperature dependence of high field 13C dynamic nuclear polarization processes with trityl radicals below 35 Kelvin From The DNP-NMR Blog: Temperature dependence of high field 13C dynamic nuclear polarization processes with trityl radicals below 35 Kelvin Walker, S.A., et al., Temperature dependence of high field 13C dynamic nuclear polarization processes with trityl radicals below 35 Kelvin. Phys. Chem. Chem. Phys., 2013. http://dx.doi.org/10.1039/C3CP51628H	nmrlearner	News from NMR blogs	0	09-06-2013 06:52 PM
High Frequency Dynamic Nuclear Polarization From The DNP-NMR Blog: High Frequency Dynamic Nuclear Polarization Ni, Q.Z., et al., High Frequency Dynamic Nuclear Polarization. Acc Chem Res, 2013. http://www.ncbi.nlm.nih.gov/pubmed/23597038	nmrlearner	News from NMR blogs	0	06-07-2013 10:42 PM
High-Field (13)C Dynamic Nuclear Polarization with a Radical Mixture From the The DNP-NMR Blog: High-Field (13)C Dynamic Nuclear Polarization with a Radical Mixture Michaelis, V.K., et al., High-Field (13)C Dynamic Nuclear Polarization with a Radical Mixture. J Am Chem Soc, 2013. http://www.ncbi.nlm.nih.gov/pubmed/23373472	nmrlearner	News from NMR blogs	0	04-15-2013 08:52 AM
Dynamic nuclear polarization at high magnetic fields in liquids Dynamic nuclear polarization at high magnetic fields in liquids July 2012 Publication year: 2012 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 64</br> </br> Graphical abstract	nmrlearner	Journal club	0	12-15-2012 09:51 AM
Dynamic nuclear polarization at high magnetic fields in liquids Dynamic nuclear polarization at high magnetic fields in liquids July 2012 Publication year: 2012 Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 64</br> </br> Graphical abstract	nmrlearner	Journal club	0	12-01-2012 06:10 PM
Dynamic nuclear polarization at high magnetic fields in liquids Dynamic nuclear polarization at high magnetic fields in liquids Publication year: 2011 Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br> C. Griesinger, M. Bennati, H.M. Vieth, C. Luchinat, G. Parigi, P. Höfer, F. Engelke, S.J. Glaser, V. Denysenkov, T.F. Prisner</br> Graphical Abstract http://ars.sciencedirect.com/content/image/1-s2.0-S0079656511000689-fx1.jpg Graphical abstract Highlights	nmrlearner	Journal club	0	03-09-2012 09:16 AM
Dynamic Nuclear Polarization at High Magnetic Fields in Liquids Dynamic Nuclear Polarization at High Magnetic Fields in Liquids Publication year: 2011 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, Available online 4 November 2011</br> C.Griesinger, M.Bennati, H.M.Vieth, C.Luchinat, G.*Parigi, ...</br> Highlights ? High field Dynamic Nuclear Polarization spectrometers for liquid samples have been constructed, working at 7, 9.2 and 14 T, respectively. ? The field dependence of the Overhauser DNP efficiency has been measured experimentally for the first time up to a field of 9.2 T and compared with experimental results from...	nmrlearner	Journal club	0	11-05-2011 09:21 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off