Rivkah Rogawski, Ivan V. Sergeyev, Yongjun Li, M. Francesca Ottaviani, Virginia Cornish, and Ann E. McDermott The Journal of Physical Chemistry B 2017 121 (6), 1169-1175
Dynamic nuclear polarization is an emerging technique for sensitizing solid-state NMR experiments by transferring polarization from electrons to nuclei. Stable biradicals, the polarization source for the cross effect mechanism, are typically codissolved at millimolar concentrations with proteins of interest. Here we describe the high-affinity biradical tag TMP-T, created by covalently linking trimethoprim, a nanomolar affinity ligand of dihydrofolate reductase (DHFR), to the biradical polarizing agent TOTAPOL. With TMP-T bound to DHFR, large enhancements of the protein spectrum are observed, comparable to when TOTAPOL is codissolved with the protein. In contrast to TOTAPOL, the tight binding TMP-T can be added stoichiometrically at radical concentrations orders of magnitude lower than in previously described preparations. Benefits of the reduced radical concentration include reduced spectral bleaching, reduced chemical perturbation of the sample, and the ability to selectively enhance signals for the protein of interest.
Dynamic Nuclear Polarization Signal Amplification as a Sensitive Probe for Specific Functionalization of Complex Paper Substrates #DNPNMR
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Dynamic Nuclear Polarization Signal Amplification as a Sensitive Probe for Specific Functionalization of Complex Paper Substrates #DNPNMR
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Gutmann, T., et al., Dynamic Nuclear Polarization Signal Amplification as a Sensitive Probe for Specific Functionalization of Complex Paper Substrates. The Journal of Physical Chemistry C, 2017. 121(7): p. 3896-3903.
http://dx.doi.org/10.1021/acs.jpcc.6b11751
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04-05-2017 02:30 PM
Dynamic Nuclear Polarization Signal Enhancement with High-Affinity Biradical Tags #DNPNMR
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Dynamic Nuclear Polarization Signal Enhancement with High-Affinity Biradical Tags #DNPNMR
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Rogawski, R., et al., Dynamic Nuclear Polarization Signal Enhancement with High-Affinity Biradical Tags. The Journal of Physical Chemistry B, 2017. 121(6): p. 1169-1175.
http://dx.doi.org/10.1021/acs.jpcb.6b09021
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03-20-2017 05:16 PM
Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy #DNPNMR
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Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy #DNPNMR
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Ravera, E., et al., Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy. ChemPhysChem, 2015. 16(13): p. 2751-2754.
https://www.ncbi.nlm.nih.gov/pubmed/26266832
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03-16-2017 04:38 AM
Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR
From The DNP-NMR Blog:
Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR
Voinov, M.A., et al., Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR. J Phys Chem B, 2015. 119(32): p. 10180-90.
http://www.ncbi.nlm.nih.gov/pubmed/26230514
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11-17-2015 02:02 AM
Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR
From The DNP-NMR Blog:
Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR
Voinov, M.A., et al., Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR. J Phys Chem B, 2015. 119(32): p. 10180-90.
http://www.ncbi.nlm.nih.gov/pubmed/26230514
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08-24-2015 06:42 PM
[NMR paper] Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR.
Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR.
Related Articles Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR.
J Phys Chem B. 2015 Jul 31;
Authors: Voinov MA, Good DB, Ward ME, Milikisiyants S, Marek A, Caporini MA, Rosay M, Munro RA, Ljumovic M, Brown LS, Ladizhansky V, Smirnov AI
Abstract
Dynamic nuclear polarization (DNP) enhances the signal in solid-state NMR of proteins by transferring polarization from...
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08-02-2015 07:10 AM
[NMR paper] Solid-state NMR on bacterial cells: selective cell wall signal enhancement and resolution improvement using dynamic nuclear polarization.
Solid-state NMR on bacterial cells: selective cell wall signal enhancement and resolution improvement using dynamic nuclear polarization.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Solid-state NMR on bacterial cells: selective cell wall signal enhancement and resolution improvement using dynamic nuclear polarization.
J Am Chem Soc. 2013 Apr 3;135(13):5105-10
Authors: Takahashi H, Ayala I, Bardet M, De Paëpe G, Simorre JP, Hediger S
Abstract
...
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10-14-2014 09:48 PM
Solid-State NMR on Bacterial Cells: Selective Cell Wall Signal Enhancement and Resolution Improvement using Dynamic Nuclear Polarization
From the The DNP-NMR Blog:
Solid-State NMR on Bacterial Cells: Selective Cell Wall Signal Enhancement and Resolution Improvement using Dynamic Nuclear Polarization
Takahashi, H., et al., Solid-State NMR on Bacterial Cells: Selective Cell Wall Signal Enhancement and Resolution Improvement using Dynamic Nuclear Polarization. J. Am. Chem. Soc., 2013.
http://dx.doi.org/10.1021/ja312501d
Dynamic nuclear polarization (DNP) enhanced solid-state nuclear magnetic resonance (NMR) has recently emerged as a powerful technique for the study of material surfaces. In this study, we demonstrate...
T1 - Dynamic Nuclear Polarization Signal Enhancement with High-Affinity Biradical Tags #DNPNMR
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