[Question from NMRWiki Q&A forum] Method for indirect 13C-1H J-couplings measurements
Method for indirect 13C-1H J-couplings measurements
Dear colleagues! 13C-1H J-couplings are essential in some areas of molecule structure research.
I discovered modern methods implemented in our software and found the following:
<ol> 13C J-resolved (recommended by Braun, Berger, Kalinovksy in 150 and more NMR experiments)
J-HMBC (once I used it, but as I remember, it would't work with J
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11-12-2011 01:40 AM
[NMR paper] Identification of protein surfaces by NMR measurements with a pramagnetic Gd(III) che
Identification of protein surfaces by NMR measurements with a pramagnetic Gd(III) chelate.
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J Am Chem Soc. 2002 Jan 23;124(3):372-3
Authors: Pintacuda G, Otting G
Gd-diethylenetriamine pentaacetic acid-bismethylamide, Gd(DTPA-BMA), is shown to be a reagent suitable for the identification of protein surfaces. Compared to the conventionally used spin-label TEMPOL, Gd(DTPA-BMA) is a stronger relaxation agent, requiring lesser concentrations...
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11-24-2010 08:49 PM
[NMR paper] Toward direct determination of conformations of protein building units from multidime
Toward direct determination of conformations of protein building units from multidimensional NMR experiments part II: a theoretical case study of formyl-L-valine amide.
Related Articles Toward direct determination of conformations of protein building units from multidimensional NMR experiments part II: a theoretical case study of formyl-L-valine amide.
Chemistry. 2001 Mar 2;7(5):1069-83
Authors: Perczel A, Császár AG
Chemical shielding anisotropy tensors have been determined for all twenty-seven characteristic conformers of For-L-Val-NH2 using...
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11-19-2010 08:32 PM
[NMR paper] In vitro NMR proton T1 measurements in peritoneal ascites.
In vitro NMR proton T1 measurements in peritoneal ascites.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--ej.iop.org-icons-external-pubmed-iop-ej.gif Related Articles In vitro NMR proton T1 measurements in peritoneal ascites.
Phys Med Biol. 1996 Mar;41(3):539-49
Authors: Yilmaz A, Tez M, Göral V, Boylu S, Kaplan A, Kavak G
The proton spin-lattice relaxation rate (1/T1) in malignant and non-malignant ascites was measured with an FT NMR spectrometer operating at 60 MHz. The mean relaxation rate in non-malignant ascites was...
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08-22-2010 02:27 PM
[NMR paper] Comparison of pH measurements made using 31P NMR and a fibreoptic pH meter.
Comparison of pH measurements made using 31P NMR and a fibreoptic pH meter.
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Authors: Jayasundar R, Hall LD, Bleehen NM
The objective of this study was to compare pH measurements made in biological samples using 31P NMR (pHNMR) with those made with a novel, dye-based fibreoptic pH measurement system (pHF), which is compatible with use in electromagnetic fields without field perturbation. Using protein-free model...
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08-21-2010 11:45 PM
[U. of Ottawa NMR Facility Blog] Faster Relaxation Time Measurements in Solids
Faster Relaxation Time Measurements in Solids
T1 relaxation times are typically measured with the inversion recovery technique. In this method the magnetization is inverted and its recovery is monitored as a function of time. For nuclei with long T1's, the measurements are very time consuming as a recycle delay of at least five times T1 must be used between scans. Typical T1's for 13C in the solid state range from several seconds to tens of minutes, so their direct measurement via the inversion recovery method could be prohibitively long.
High resolution 13C solid state NMR spectra of...
Systems of Units of Measurements
Linear Mode
