Studies on the MxiH protein in T3SS needles using DNP-enhanced ssNMR spectroscopy #DNPNMR

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Studies on the MxiH protein in T3SS needles using DNP-enhanced ssNMR spectroscopy #DNPNMR

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NMR processing:

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Structure from NMR restraints:

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Fragment-based:

Rosetta-NMR (Robetta)

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GeNMR

I-TASSER

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Amber

Structure from chemical shifts:

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Torsion angles from chemical shifts:

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TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

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d2D

PECAN

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RCI

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HADDOCK

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Methyl S2

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Shiftx2

Sparta+

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CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

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Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

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CCPN

From NMR-STAR 3.1

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DisMeta

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01-30-2017, 03:31 PM

nmrlearner

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Studies on the MxiH protein in T3SS needles using DNP-enhanced ssNMR spectroscopy #DNPNMR

From The DNP-NMR Blog:

Studies on the MxiH protein in T3SS needles using DNP-enhanced ssNMR spectroscopy #DNPNMR

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Fricke, P., et al., Studies on the MxiH protein in T3SS needles using DNP-enhanced ssNMR spectroscopy. ChemPhysChem, 2014. 15(1): p. 57-60.

https://www.ncbi.nlm.nih.gov/pubmed/24282046

Bacterial T3SS needles formed by the protein MxiH are studied using DNP-enhanced ssNMR spectroscopy at 14.1 T (600 MHz). This technique provides spectra of good resolution, allowing us to draw conclusions about the protein dynamics. With the obtained signal enhancement, samples of limited quantity now get within reach of ssNMR studies.
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