Milshteyn, Eugene, Galen D. Reed, Jeremy W. Gordon, Cornelius von Morze, Peng Cao, Shuyu Tang, Andrew P. Leynes, Peder E.Z. Larson, and Daniel B. Vigneron. “Simultaneous T1 and T2 Mapping of Hyperpolarized 13C Compounds Using the BSSFP Sequence.” Journal of Magnetic Resonance 312 (March 2020): 106691.
As in conventional 1H MRI, T1 and T2 relaxation times of hyperpolarized (HP) 13C nuclei can provide important biomedical information. Two new approaches were developed for simultaneous T1 and T2 mapping of HP 13C probes based on balanced steady state free precession (bSSFP) acquisitions: a method based on sequential T1 and T2 mapping modules, and a model-based joint T1/T2 approach analogous to MR fingerprinting. These new methods were tested in simulations, HP 13C phantoms, and in vivo in normal Sprague-Dawley rats. Non-localized T1 values, low flip angle EPI T1 maps, bSSFP T2 maps, and Bloch-Siegert B1 maps were also acquired for comparison. T1 and T2 maps acquired using both approaches were in good agreement with both literature values and data from comparative acquisitions. Multiple HP 13C compounds were successfully mapped, with their relaxation time parameters measured within heart, liver, kidneys, and vasculature in one acquisition for the first time.
Determination of binding affinities using hyperpolarized NMR with simultaneous 4-channel detection
From The DNP-NMR Blog:
Determination of binding affinities using hyperpolarized NMR with simultaneous 4-channel detection
Kim, Yaewon, Mengxiao Liu, and Christian Hilty. “Determination of Binding Affinities Using Hyperpolarized NMR with Simultaneous 4-Channel Detection.” Journal of Magnetic Resonance 295 (October 2018): 80–86.
https://doi.org/10.1016/j.jmr.2018.08.002.
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11-25-2018 06:02 AM
TROSY pulse sequence for simultaneous measurement of the 15 N R 1 and { 1 H}â?? 15 N NOE in deuterated proteins
TROSY pulse sequence for simultaneous measurement of the 15 N R 1 and { 1 H}â?? 15 N NOE in deuterated proteins
Abstract
A TROSY-based NMR experiment is described for simultaneous measurement of the 15N longitudinal relaxation rate constant R1 and the {1H}â??15N nuclear Overhauser enhancement. The experiment is based on the observation that the TROSY mixing pulse sequence element symmetrically exchanges 1H and 15N magnetizations. The accuracy of the proposed technique is validated by comparison to independent measurements of both relaxation parameters...
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04-16-2018 11:30 AM
[NMR paper] 1H-detected MAS solid-state NMR experiments enable the simultaneous mapping of rigid and dynamic domains of membrane proteins.
1H-detected MAS solid-state NMR experiments enable the simultaneous mapping of rigid and dynamic domains of membrane proteins.
1H-detected MAS solid-state NMR experiments enable the simultaneous mapping of rigid and dynamic domains of membrane proteins.
J Magn Reson. 2017 Dec;285:101-107
Authors: Gopinath T, Nelson SED, Veglia G
Abstract
Magic angle spinning (MAS) solid-state NMR (ssNMR) spectroscopy is emerging as a unique method for the atomic resolution structure determination of native membrane proteins in lipid bilayers....
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11-28-2017 04:45 PM
[NMR paper] 1H-detected MAS solid-state NMR experiments enable the simultaneous mapping of rigid and dynamic domains of membrane proteins
1H-detected MAS solid-state NMR experiments enable the simultaneous mapping of rigid and dynamic domains of membrane proteins
Publication date: December 2017
Source:Journal of Magnetic Resonance, Volume 285</br>
Author(s): T. Gopinath, Sarah E.D. Nelson, Gianluigi Veglia</br>
Magic angle spinning (MAS) solid-state NMR (ssNMR) spectroscopy is emerging as a unique method for the atomic resolution structure determination of native membrane proteins in lipid bilayers. Although 13C-detected ssNMR experiments continue to play a major role, recent technological...
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11-24-2017 05:56 PM
Simultaneous hyperpolarized (13)C-pyruvate MRI and (18)F-FDG-PET in cancer (hyperPET): feasibility of a new imaging concept using a clinical PET/MRI scanner
From The DNP-NMR Blog:
Simultaneous hyperpolarized (13)C-pyruvate MRI and (18)F-FDG-PET in cancer (hyperPET): feasibility of a new imaging concept using a clinical PET/MRI scanner
Gutte, H., et al., Simultaneous hyperpolarized (13)C-pyruvate MRI and (18)F-FDG-PET in cancer (hyperPET): feasibility of a new imaging concept using a clinical PET/MRI scanner. American Journal of Nuclear Medicine and Molecular Imaging, 2015. 5(1): p. 38-45.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4299777/
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05-14-2015 04:52 AM
Apparent rate constant mapping using hyperpolarized [1–13C]pyruvate
From The DNP-NMR Blog:
Apparent rate constant mapping using hyperpolarized pyruvate
Khegai, O., et al., Apparent rate constant mapping using hyperpolarized pyruvate. NMR in Biomedicine, 2014. 27(10): p. 1256-1265.
http://dx.doi.org/10.1002/nbm.3174
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01-26-2015 03:48 PM
[NMR paper] Sequence-specific NMR assignment of proteins by global fragment mapping with the prog
Sequence-specific NMR assignment of proteins by global fragment mapping with the program MAPPER.
Related Articles Sequence-specific NMR assignment of proteins by global fragment mapping with the program MAPPER.
J Biomol NMR. 2000 Oct;18(2):129-37
Authors: Güntert P, Salzmann M, Braun D, Wüthrich K
A new program, MAPPER, for semiautomatic sequence-specific NMR assignment in proteins is introduced. The program uses an input of short fragments of sequentially neighboring residues, which have been assembled based on sequential NMR connectivities...
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11-19-2010 08:29 PM
Simultaneous detection of amide and methyl correlations using a time shared NMR experiment: application to binding epitope mapping
Simultaneous detection of amide and methyl correlations using a time shared NMR experiment: application to binding epitope mapping
Peter Würtz, Olli Aitio, Maarit Hellman and Perttu Permi
Journal of Biomolecular NMR; 2007; 39(2) pp 97 - 105
Abstract:
Simultaneous recording of different NMR parameters is an efficient way to reduce the overall experimental time and speed up structural studies of biological macromolecules. This can especially be beneficial in the case of fast NMR-based drug screening applications or for collecting NOE restraints, where prohibitively long data collection...
Simultaneous T1 and T2 mapping of hyperpolarized 13C compounds using the bSSFP sequence #DNPNMR
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