Sensitivity-enhanced NMR reveals alterations in protein structure by cellular milieus

		BioNMR > NMR community > News from NMR blogs
Sensitivity-enhanced NMR reveals alterations in protein structure by cellular milieus

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-23-2016, 12:44 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Sensitivity-enhanced NMR reveals alterations in protein structure by cellular milieus

From The DNP-NMR Blog:

Sensitivity-enhanced NMR reveals alterations in protein structure by cellular milieus

Frederick, K.K., et al., Sensitivity-enhanced NMR reveals alterations in protein structure by cellular milieus. Cell, 2015. 163(3): p. 620-8.

http://www.ncbi.nlm.nih.gov/pubmed/26456111

Biological processes occur in complex environments containing a myriad of potential interactors. Unfortunately, limitations on the sensitivity of biophysical techniques normally restrict structural investigations to purified systems, at concentrations that are orders of magnitude above endogenous levels. Dynamic nuclear polarization (DNP) can dramatically enhance the sensitivity of nuclear magnetic resonance (NMR) spectroscopy and enable structural studies in biologically complex environments. Here, we applied DNP NMR to investigate the structure of a protein containing both an environmentally sensitive folding pathway and an intrinsically disordered region, the yeast prion protein Sup35. We added an exogenously prepared isotopically labeled protein to deuterated lysates, rendering the biological environment "invisible" and enabling highly efficient polarization transfer for DNP. In this environment, structural changes occurred in a region known to influence biological activity but intrinsically disordered in purified samples. Thus, DNP makes structural studies of proteins at endogenous levels in biological contexts possible, and such contexts can influence protein structure.

Go to The DNP-NMR Blog for more info.

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Into the fold: Sensitivity-enhanced NMR Into the fold: Sensitivity-enhanced NMR http://www.spectroscopynow.com/common/images/thumbnails/15061c31a88.jpgResearchers at Massachusetts Institute of Technology have used sensitivity-enhanced nuclear magnetic resonance (NMR) spectroscopy to analyse the structure that a yeast protein forms as it interacts with other proteins in a cell opening up new insights into protein folding and misfolding. Read the rest at Spectroscopynow.com	nmrlearner	General	0	10-15-2015 12:19 PM
[NMR paper] Sensitivity-Enhanced NMR Reveals Alterations in Protein Structure by Cellular Milieus. Sensitivity-Enhanced NMR Reveals Alterations in Protein Structure by Cellular Milieus. Sensitivity-Enhanced NMR Reveals Alterations in Protein Structure by Cellular Milieus. Cell. 2015 Oct 7; Authors: Frederick KK, Michaelis VK, Corzilius B, Ong TC, Jacavone AC, Griffin RG, Lindquist S Abstract Biological processes occur in complex environments containing a myriad of potential interactors. Unfortunately, limitations on the sensitivity of biophysical techniques normally restrict structural investigations to purified systems, at...	nmrlearner	Journal club	0	10-13-2015 06:03 PM
Enhanced-sensitivity NMR could reveal new clues to how proteins fold - Phys.Org Enhanced-sensitivity NMR could reveal new clues to how proteins fold - Phys.Org http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcRv5kcqepdG7qJM0UMWGlFFd6B7LANvsM9YhElwPbgnXWRAEWGTHs3JS_oQLgMHS8kZCRwpmbo Phys.Org <img alt="" height="1" width="1"> Enhanced-sensitivity NMR could reveal new clues to how proteins fold Phys.Org Until now, it has been difficult to fully characterize the different structures that proteins can take on in their natural environments. However, using a new technique known as sensitivity-enhanced nuclear magnetic resonance (NMR), MIT researchers have ... ...	nmrlearner	Online News	0	10-09-2015 04:49 PM
Sensitivity-Enhanced NMR Reveals Alterations in Protein Structure by Cellular Milieus Sensitivity-Enhanced NMR Reveals Alterations in Protein Structure by Cellular Milieus Publication date: Available online 8 October 2015 Source:Cell</br> Author(s): KendraK. Frederick, VladimirK. Michaelis, Björn Corzilius, Ta-Chung Ong, AngelaC. Jacavone, RobertG. Griffin, Susan Lindquist</br> Biological processes occur in complex environments containing a myriad of potential interactors. Unfortunately, limitations on the sensitivity of biophysical techniques normally restrict structural investigations to purified systems, at concentrations that are...	nmrlearner	Journal club	0	10-09-2015 03:05 AM
HyperBIRD: A Sensitivity-Enhanced Approach to Collecting Homonuclear-Decoupled Proton NMR Spectra From The DNP-NMR Blog: HyperBIRD: A Sensitivity-Enhanced Approach to Collecting Homonuclear-Decoupled Proton NMR Spectra Donovan, K.J. and L. Frydman, HyperBIRD: A Sensitivity-Enhanced Approach to Collecting Homonuclear-Decoupled Proton NMR Spectra. Angew Chem Int Ed Engl, 2014: p. n/a-n/a. http://www.ncbi.nlm.nih.gov/pubmed/25256418	nmrlearner	News from NMR blogs	0	01-09-2015 03:58 PM
[NMR paper] Sensitivity-enhanced static 15N NMR of solids by 1h indirect detection. Sensitivity-enhanced static 15N NMR of solids by 1h indirect detection. Related Articles Sensitivity-enhanced static 15N NMR of solids by 1h indirect detection. J Magn Reson. 2001 May;150(1):43-8 Authors: Hong M, Yamaguchi S A method for enhancing the sensitivity of 15N spectra of nonspinning solids through 1H indirect detection is introduced. By sampling the 1H signals in the windows of a pulsed spin-lock sequence, high-sensitivity 1H spectra can be obtained in two-dimensional (2D) spectra whose indirect dimension yields the 15N chemical...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
[NMR paper] Sensitivity enhanced NMR spectroscopy by quenching scalar coupling mediated relaxatio Sensitivity enhanced NMR spectroscopy by quenching scalar coupling mediated relaxation: application to the direct observation of hydrogen bonds in 13C/15N-labeled proteins. Related Articles Sensitivity enhanced NMR spectroscopy by quenching scalar coupling mediated relaxation: application to the direct observation of hydrogen bonds in 13C/15N-labeled proteins. J Biomol NMR. 2000 May;17(1):55-61 Authors: Liu A, Hu W, Qamar S, Majumdar A In this paper, we demonstrate that the sensitivity of triple-resonance NMR experiments can be enhanced...	nmrlearner	Journal club	0	11-18-2010 09:15 PM
Structure of key protein for cellular signal transduction elucidated - News-Medical.n Structure of key protein for cellular signal transduction elucidated - News-Medical.net <img alt="" height="1" width="1" /> Structure of key protein for cellular signal transduction elucidated News-Medical.net Using NMR spectroscopy, Professor Michael Sattler and his team elucidated the spatial structure of the Qua1 region of Sam68, which is responsible for the ... and more » Read here	nmrlearner	Online News	0	09-10-2010 12:58 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off