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Viennet, T., et al., Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization. Angew Chem Int Ed Engl, 2016. 55(36): p. 10746-50.Viennet, T., et al., Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization. Angew Chem Int Ed Engl, 2016. 55(36): p. 10746-50.
Nuclear magnetic resonance (NMR) spectroscopy has the intrinsic capabilities to investigate proteins in native environments. In general, however, NMR relies on non-natural protein purity and concentration to increase the desired signal over the background. We here report on the efficient and specific hyperpolarization of low amounts of a target protein in a large isotope-labeled background by combining dynamic nuclear polarization (DNP) and the selectivity of protein interactions. Using a biradical-labeled ligand, we were able to direct the hyperpolarization to the protein of interest, maintaining comparable signal enhancement with about 400-fold less radicals than conventionally used. We could selectively filter out our target protein directly from crude cell lysate obtained from only 8 mL of fully isotope-enriched cell culture. Our approach offers effective means to study proteins with atomic resolution in increasingly native concentrations and environments.
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Phenylazide Hybrid-Silica - Polarization Platform for Dynamic Nuclear Polarization at Cryogenic Temperatures #DNPNMR
From The DNP-NMR Blog:
Phenylazide Hybrid-Silica - Polarization Platform for Dynamic Nuclear Polarization at Cryogenic Temperatures #DNPNMR
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Grüning, W.R., et al., Phenylazide Hybrid-Silica - Polarization Platform for Dynamic Nuclear Polarization at Cryogenic Temperatures. Helvetica Chimica Acta, 2016: p. n/a-n/a.
http://dx.doi.org/10.1002/hlca.201600122
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11-19-2016 08:35 PM
Hyperpolarization of Frozen Hydrocarbon Gases by Dynamic Nuclear Polarization at 1.2 K #DNPNMR
From The DNP-NMR Blog:
Hyperpolarization of Frozen Hydrocarbon Gases by Dynamic Nuclear Polarization at 1.2 K #DNPNMR
Vuichoud, B., et al., Hyperpolarization of Frozen Hydrocarbon Gases by Dynamic Nuclear Polarization at 1.2 K. J Phys Chem Lett, 2016. 7(16): p. 3235-9.
https://www.ncbi.nlm.nih.gov/pubmed/27483034
Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies #DNPNMR
From The DNP-NMR Blog:
Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies #DNPNMR
Gupta, R., et al., Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies. J Phys Chem B, 2016. 120(2): p. 329-39.
http://www.ncbi.nlm.nih.gov/pubmed/26709853
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05-25-2016 02:33 PM
Essentials of Dynamic Nuclear Polarization #DNPNMR
From The DNP-NMR Blog:
Essentials of Dynamic Nuclear Polarization #DNPNMR
Dear Colleague,
It is my pleasure to announce that a limited edition of
`Essentials of Dynamic Nuclear Polarization'
is now available at the UK branch of Amazon: www.amazon.co.uk
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News from NMR blogs
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05-25-2016 02:30 AM
[NMR paper] Solid-state NMR on bacterial cells: selective cell wall signal enhancement and resolution improvement using dynamic nuclear polarization.
Solid-state NMR on bacterial cells: selective cell wall signal enhancement and resolution improvement using dynamic nuclear polarization.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Solid-state NMR on bacterial cells: selective cell wall signal enhancement and resolution improvement using dynamic nuclear polarization.
J Am Chem Soc. 2013 Apr 3;135(13):5105-10
Authors: Takahashi H, Ayala I, Bardet M, De Paëpe G, Simorre JP, Hediger S
Abstract
...
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10-14-2014 09:48 PM
Hyperpolarization of deuterated metabolites via remote cross-polarization and dissolution dynamic nuclear polarization
From The DNP-NMR Blog:
Hyperpolarization of deuterated metabolites via remote cross-polarization and dissolution dynamic nuclear polarization
Vuichoud, B., et al., Hyperpolarization of deuterated metabolites via remote cross-polarization and dissolution dynamic nuclear polarization. J Phys Chem B, 2014. 118(5): p. 1411-5.
http://www.ncbi.nlm.nih.gov/pubmed/24397585
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04-02-2014 11:54 PM
Solid-State NMR on Bacterial Cells: Selective Cell Wall Signal Enhancement and Resolution Improvement using Dynamic Nuclear Polarization
From the The DNP-NMR Blog:
Solid-State NMR on Bacterial Cells: Selective Cell Wall Signal Enhancement and Resolution Improvement using Dynamic Nuclear Polarization
Takahashi, H., et al., Solid-State NMR on Bacterial Cells: Selective Cell Wall Signal Enhancement and Resolution Improvement using Dynamic Nuclear Polarization. J. Am. Chem. Soc., 2013.
http://dx.doi.org/10.1021/ja312501d
Dynamic nuclear polarization (DNP) enhanced solid-state nuclear magnetic resonance (NMR) has recently emerged as a powerful technique for the study of material surfaces. In this study, we demonstrate...
Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization #DNPNMR
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