Meerten, S.G.J. van, G.E. Janssen, and A.P.M. Kentgens. “Rapid-Melt DNP for Multidimensional and Heteronuclear High-Field NMR Experiments.” Journal of Magnetic Resonance 310 (January 2020): 106656.
Low sensitivity is the main limitation of NMR for efficient chemical analysis of mass-limited samples. Hyperpolarization techniques such as Dynamic Nuclear Polarization (DNP) have greatly improved the efficiency of NMR experiments. In this manuscript, we demonstrate a 400 MHz rapid-melt DNP setup. With this setup it is possible to perform liquid-state NMR experiments with solid-state DNP enhancement at high magnetic field. Sample volumes of 100 nL in fused-silica capillaries are detected using a stripline microcoil. Due to the small heat capacity of these samples it is possible to melt them with relatively low relaxation losses. With this 400 MHz setup, proton enhancements of up to À175 have been obtained in the liquid-state. The probe is double tuned, so it can be used for heteronuclear DNP-NMR and since the sample composition does not change during the experiment, it is possible to perform signal averaging and multidimensional experiments. This type of rapid-melt DNP setup thus allows for most types of liquid-state NMR experiments to be combined with efficient solid-state DNP. This makes rapid-melt DNP an interesting method for high-throughput chemical analysis of mass-limited samples.
Mechanism of spontaneous polarization transfer in high-field SABRE experiments
From The DNP-NMR Blog:
Mechanism of spontaneous polarization transfer in high-field SABRE experiments
Knecht, S., et al., Mechanism of spontaneous polarization transfer in high-field SABRE experiments. Journal of Magnetic Resonance, 2018. 287: p. 74-81.
https://doi.org/10.1016/j.jmr.2017.12.018
nmrlearner
News from NMR blogs
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03-06-2018 03:16 AM
Solid Effect DNP in a Rapid-melt setup #DNPNMR
From The DNP-NMR Blog:
Solid Effect DNP in a Rapid-melt setup #DNPNMR
van Bentum, P.J.M., et al., Solid Effect DNP in a Rapid-melt setup. J. Magn. Reson., 2016. 263: p. 126-135.
http://dx.doi.org/10.1016/j.jmr.2015.12.023
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News from NMR blogs
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07-04-2016 11:25 PM
Rapid-melt Dynamic Nuclear Polarization
From The DNP-NMR Blog:
Rapid-melt Dynamic Nuclear Polarization
Sharma, M., et al., Rapid-melt Dynamic Nuclear Polarization. J Magn Reson, 2015. 258: p. 40-8.
http://www.ncbi.nlm.nih.gov/pubmed/26225439
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News from NMR blogs
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12-12-2015 03:39 AM
Journal Highlight: Next-generation heteronuclear decoupling for high-field biomolecular NMR spectroscopy
Journal Highlight: Next-generation heteronuclear decoupling for high-field biomolecular NMR spectroscopy
http://www.spectroscopynow.com/common/images/thumbnails/145c16186a0.jpgA novel low-power, broadband heteronuclear decoupling pulse is presented that generates minimal, ultra-low sidebands for ultra-high-field NMR spectroscopy.
Read the rest at Spectroscopynow.com
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General
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05-05-2014 01:57 PM
[NMR paper] Next-Generation Heteronuclear Decoupling for High-Field Biomolecular NMR Spectroscopy.
Next-Generation Heteronuclear Decoupling for High-Field Biomolecular NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Next-Generation Heteronuclear Decoupling for High-Field Biomolecular NMR Spectroscopy.
Angew Chem Int Ed Engl. 2014 Mar 12;
Authors: Schilling F, Warner LR, Gershenzon NI, Skinner TE, Sattler M, Glaser SJ
Abstract
Ultra-high-field NMR spectroscopy requires an increased bandwidth for...
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Journal club
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03-14-2014 07:34 PM
Postdoctoral positions "high field ?MAS-DNP- NMR” and “Rapid melt DNP"
From The DNP-NMR Blog:
Postdoctoral positions "high field ?MAS-DNP- NMR” and “Rapid melt DNP"
Two postdoc positions for “DNP-enhanced NMR” both in the solid and liquid state are available immediately at the Solid State NMR group of the Institute of Molecules and Materials at the Radboud University of Nijmegen.
The solid NMR group at the Radboud University specializes in developing innovative technology to perform NMR spectroscopy on very small samples volumes both in the solid and in the liquid state. In the solid phase one can obtain ultrahigh proton decoupling fields (above 1...
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News from NMR blogs
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06-21-2013 12:16 AM
[NMR paper] A novel microfluidic rapid freeze-quench device for trapping reactions intermediates for high field EPR analysis
A novel microfluidic rapid freeze-quench device for trapping reactions intermediates for high field EPR analysis
Available online 5 March 2013
Publication year: 2013
Source:Journal of Magnetic Resonance</br>
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Rapid freeze quench electron paramagnetic resonance (RFQ)-EPR is a method for trapping short lived intermediates in chemical reactions and subjecting them to EPR spectroscopy investigation for their characterization. Two (or more) reacting components are mixed at room temperature and after some delay the mixture is sprayed into a cold trap and transferred into...
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Journal club
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03-06-2013 04:33 PM
[NMR paper] High-Resolution Heteronuclear Multidimensional NMR of Proteins in Living Insect Cells Using a Baculovirus Protein Expression System.
High-Resolution Heteronuclear Multidimensional NMR of Proteins in Living Insect Cells Using a Baculovirus Protein Expression System.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles High-Resolution Heteronuclear Multidimensional NMR of Proteins in Living Insect Cells Using a Baculovirus Protein Expression System.
J Am Chem Soc. 2013 Jan 27;
Authors: Hamatsu J, O'Donovan D, Tanaka T, Shirai T, Hourai Y, Mikawa T, Ikeya T, Mishima M, Boucher W, Smith BO, Laue ED, Shirakawa M, Ito Y
...
Rapid-melt DNP for multidimensional and heteronuclear high-field NMR experiments #DNPNMR
Linear Mode
