Chemical sensors informing about their local environment are of widespread use for chemical analysis. A thorough understanding of the sensor signaling is fundamental to data analysis and interpretation, and a requirement for technological applications. Here, sensors explored for the recognition and display of biomolecular and cellular markers by magnetic resonance and composed of host molecules for xenon atoms are considered. These host-guest systems are analytically powerful and also function as contrast agents in imaging applications. Using nuclear spin hyperpolarization of 129Xe and chemical exchange saturation transfer the detection sensitivity is orders of magnitude enhanced in comparison to conventional 1H NMR. The sensor signaling reflects this rather complex genesis, furthering the mere qualitative interpretation of biosensing data; to harvest the potential of the approach, however, a detailed numerical account is desired. To this end, we introduce a comprehensive expression that maps the sensor detection quantitatively by integration of the hyperpolarization generation and relaxation with the host-xenon exchange dynamics. As demonstrated for the host molecule and well-established biosensor cryptophane-A, this model reveals a distinguished maximum in sensor signaling and exerts control over experimentation by dedicated adjustments of both the amount of xenon and the duration of the saturation transfer applied in a measurement, for example to capitalize on investigations at the detection limit. Furthermore, usage of the model for data analysis makes the quantification of the sensor concentration in the nanomolar range possible. The approach is readily applicable in investigations using cryptophane-A and is straightaway adaptable to other sensor designs for extension of the field of xenon based biosensing.
p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica}
Rotaxane probes for protease detection by 129Xe hyperCEST NMR
From The DNP-NMR Blog:
Rotaxane probes for protease detection by 129Xe hyperCEST NMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Slack, C.C., et al., Rotaxane probes for protease detection by 129Xe hyperCEST NMR. Chem Commun (Camb), 2017. 53(6): p. 1076-1079.
https://www.ncbi.nlm.nih.gov/pubmed/28044166
nmrlearner
News from NMR blogs
0
05-03-2017 07:03 PM
Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically ... - Dove Medical Press
Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically ... - Dove Medical Press
<img alt="" height="1" width="1">
Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically ...
Dove Medical Press
Here, we report a highly sensitive magnetic-based biosensing system that is composed of a miniaturized nuclear magnetic resonance (NMR) device and magnetically engineered nanoferrite particles (NFPs). The sensing performance, also identified as the ...
Read here
nmrlearner
Online News
0
11-19-2016 08:35 PM
Modular 129Xe NMR biosensor for MRI applications [Biophysics and Computational Biology]
Modular 129Xe NMR biosensor for MRI applications
Rose, H. M., Witte, C., Rossella, F., Klippel, S., Freund, C., Schroder, L....
Date: 2014-08-12
Magnetic resonance imaging (MRI) is seriously limited when aiming for visualization of targeted contrast agents. Images are reconstructed from the weak diamagnetic properties of the sample and require an abundant molecule like water as the reporter. Micromolar to millimolar concentrations of conventional contrast agents are needed to generate image contrast,... Read More
PNAS:
Number: 32
nmrlearner
Journal club
0
08-13-2014 07:49 AM
[NMR paper] Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations.
Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations.
Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations.
Proc Natl Acad Sci U S A. 2014 Jul 28;
Authors: Rose HM, Witte C, Rossella F, Klippel S, Freund C, Schröder L
Abstract
Magnetic resonance imaging (MRI) is seriously limited when aiming for visualization of targeted contrast agents. Images are reconstructed from the weak diamagnetic...
nmrlearner
Journal club
0
07-30-2014 10:22 AM
Post-doc in hyperpolarized 129Xe MRI - CEA Saclay, France
From The DNP-NMR Blog:
Post-doc in hyperpolarized 129Xe MRI - CEA Saclay, France
From the Ampere Magnetic Resonance List:
A project untitled: « Polarized Xenon-MRI for diagnosis and follow up of Chest Tumors » is granted by the French programme « Projets de recherche dans le domaine de la physique, des mathématiques ou des sciences de l'ingénieur appliqués au cancer ».
nmrlearner
News from NMR blogs
0
05-16-2014 08:06 PM
Optimal variable flip angle schemes for dynamic acquisition of exchanging hyperpolarized substrates
From The DNP-NMR Blog:
Optimal variable flip angle schemes for dynamic acquisition of exchanging hyperpolarized substrates
Xing, Y., et al., Optimal variable flip angle schemes for dynamic acquisition of exchanging hyperpolarized substrates. J. Magn. Reson., 2013. 234(0): p. 75-81.
http://dx.doi.org/10.1016/j.jmr.2013.06.003
nmrlearner
News from NMR blogs
0
11-21-2013 01:14 AM
[NMR paper] Molecular imaging of cancer cells using a bacteriophage-based 129Xe NMR biosensor.
Molecular imaging of cancer cells using a bacteriophage-based 129Xe NMR biosensor.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Molecular imaging of cancer cells using a bacteriophage-based 129Xe NMR biosensor.
Angew Chem Int Ed Engl. 2013 Apr 26;52(18):4849-53
Authors: Palaniappan KK, Ramirez RM, Bajaj VS, Wemmer DE, Pines A, Francis MB
PMID: 23554263
nmrlearner
Journal club
0
10-19-2013 03:22 PM
[NMR paper] Detection and characterization of xenon-binding sites in proteins by 129Xe NMR spectr
Detection and characterization of xenon-binding sites in proteins by 129Xe NMR spectroscopy.
Related Articles Detection and characterization of xenon-binding sites in proteins by 129Xe NMR spectroscopy.
J Mol Biol. 2002 Sep 13;322(2):425-40
Authors: Rubin SM, Lee SY, Ruiz EJ, Pines A, Wemmer DE
Xenon-binding sites in proteins have led to a number of applications of xenon in biochemical and structural studies. Here we further develop the utility of 129Xe NMR in characterizing specific xenon-protein interactions. The sensitivity of the 129Xe...
Quantitative biosensor detection by chemically exchanging hyperpolarized 129Xe
Linear Mode
