Pyruvate membrane crossing and its lactate dehydrogenase-mediated conversion to lactate in cells featuring different levels of expression of membrane monocarboxylate transporters (MCT4) were probed by dissolution dynamic nuclear polarization-enhanced NMR. Hyperpolarized 13 C-1-labeled pyruvate was transferred to suspensions of rodent tumor cell carcinoma, cell line 39. The pyruvate-to-lactate conversion rate monitored by dissolution dynamic nuclear polarization-NMR in carcinoma cells featuring native MCT4 expression level was lower than the rate observed for cells in which the human MCT4 gene was overexpressed. The enzymatic activity of lactate dehydrogenase was also assessed in buffer solutions, following the real-time pyruvate-to-lactate conversion speeds at different enzyme concentrations. Copyright (c) 2016 John Wiley & Sons, Ltd.
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[NMR paper] Micelles, Bicelles, and Nanodiscs: Comparing the Impact of Membrane Mimetics on Membrane Protein Backbone Dynamics
Micelles, Bicelles, and Nanodiscs: Comparing the Impact of Membrane Mimetics on Membrane Protein Backbone Dynamics
Detergents are often used to investigate the structure and dynamics of membrane proteins. Whereas the structural integrity seems to be preserved in detergents for many membrane proteins, their functional activity is frequently compromised, but can be restored in a lipid environment. Herein we show with per-residue resolution that while OmpX forms a stable ?-barrel in DPC detergent micelles, DHPC/DMPC bicelles, and DMPC nanodiscs, the pico- to nanosecond and micro- to...
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Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization
From The DNP-NMR Blog:
Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization
Yamamoto, K., et al., Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization. Biochimica et Biophysica Acta (BBA) - Biomembranes, 2015. 1848(1, Part B): p. 342-349.
http://dx.doi.org/10.1016/j.bbamem.2014.07.008
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05-04-2015 04:19 PM
Long-Lived States of Magnetically Equivalent Spins Populated by Dissolution-DNP and Revealed by Enzymatic Reactions
From The DNP-NMR Blog:
Long-Lived States of Magnetically Equivalent Spins Populated by Dissolution-DNP and Revealed by Enzymatic Reactions
Bornet, A., et al., Long-Lived States of Magnetically Equivalent Spins Populated by Dissolution-DNP and Revealed by Enzymatic Reactions. Chemistry, 2014. 20(51): p. 17113-8.
http://www.ncbi.nlm.nih.gov/pubmed/25346515
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12-17-2014 09:43 PM
Cellular Solid-State NMR Investigation of a Membrane Protein Using Dynamic Nuclear Polarization
Cellular Solid-State NMR Investigation of a Membrane Protein Using Dynamic Nuclear Polarization
Publication date: Available online 11 July 2014
Source:Biochimica et Biophysica Acta (BBA) - Biomembranes</br>
Author(s): Kazutoshi Yamamoto , Marc A. Caporini , Sang-Choul Im , Lucy Waskell , Ayyalusamy Ramamoorthy</br>
While an increasing number of structural biology studies successfully demonstrate the power of high-resolution structures and dynamics of membrane proteins in fully understanding their function, there is considerable interest in developing NMR...
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07-12-2014 04:28 AM
HistoneH3 and H4 N-Terminal Tails in NucleosomeArrays at Cellular Concentrations Probed by Magic Angle Spinning NMRSpectroscopy
HistoneH3 and H4 N-Terminal Tails in NucleosomeArrays at Cellular Concentrations Probed by Magic Angle Spinning NMRSpectroscopy
Min Gao, Philippe S. Nadaud, Morgan W. Bernier, Justin A. North, P. Chris Hammel, Michael G. Poirier and Christopher P. Jaroniec
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja407526s/aop/images/medium/ja-2013-07526s_0002.gif
Journal of the American Chemical Society
DOI: 10.1021/ja407526s
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/KRNVFRDIOnY
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10-08-2013 01:05 AM
[NMR paper] Histone H3 and H4 N-Terminal Tails in Nucleosome Arrays at Cellular Concentrations Probed by Magic Angle Spinning NMR Spectroscopy.
Histone H3 and H4 N-Terminal Tails in Nucleosome Arrays at Cellular Concentrations Probed by Magic Angle Spinning NMR Spectroscopy.
Histone H3 and H4 N-Terminal Tails in Nucleosome Arrays at Cellular Concentrations Probed by Magic Angle Spinning NMR Spectroscopy.
J Am Chem Soc. 2013 Oct 2;
Authors: Gao M, Nadaud PS, Bernier MW, North JA, Hammel PC, Poirier MG, Jaroniec CP
Abstract
Chromatin is a supramolecular assembly of DNA and histone proteins, organized into nucleosome repeat units. The dynamics of chromatin organization...
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10-04-2013 05:23 PM
[NMR paper] NMR monitoring of accumulation and folding of 15N-labeled protein overexpressed in Pi
NMR monitoring of accumulation and folding of 15N-labeled protein overexpressed in Pichia pastoris.
Related Articles NMR monitoring of accumulation and folding of 15N-labeled protein overexpressed in Pichia pastoris.
Protein Expr Purif. 2001 Jul;22(2):318-24
Authors: de Lamotte F, Boze H, Blanchard C, Klein C, Moulin G, Gautier MF, Delsuc MA
Postgenomic studies have led to an increasing demand for isotope-labeled proteins. We present a method for producing large quantities of truly native (15)N-labeled protein. Based on the secretion...
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11-19-2010 08:44 PM
[NMR paper] An NMR study of cellular phosphates and membrane transport in renal proximal tubules.
An NMR study of cellular phosphates and membrane transport in renal proximal tubules.
Related Articles An NMR study of cellular phosphates and membrane transport in renal proximal tubules.
Am J Physiol. 1995 Mar;268(3 Pt 2):F375-84
Authors: Chobanian MC, Anderson ME, Brazy PC
Technical limitations in the measurement of cellular phosphates have hindered studies of interrelationships between cellular Pi, its transport, and its metabolism in renal proximal tubule (PT) cells. We have developed a noninvasive 31P-nuclear magnetic resonance (NMR)...
Pyruvate cellular uptake and enzymatic conversion probed by dissolution DNP-NMR: the impact of overexpressed membrane transporters
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