Dissolution dynamic nuclear polarization (DNP) provides a way to tremendously improve the sensitivity of nuclear magnetic resonance experiments. Once the spins are hyperpolarized by dissolution DNP, the radicals used as polarizing agents become undesirable since their presence is an additional source of nuclear spin relaxation and their toxicity might be an issue. This study demonstrates the feasibility of preparing a hyperpolarized [1-(13) C]2-methylpropan-2-ol (tert-butanol) solution free of persistent radicals by using spin-labeled thermoresponsive hydrophilic polymer networks as polarizing agents. The hyperpolarized (13) C signal can be detected for up to 5 min before the spins fully relax to their thermal equilibrium. This approach extends the applicability of spin-labeled thermoresponsive hydrogel to the dissolution DNP field and highlights its potential as polarizing agent for preparing neat slowly relaxing contrast agents. The hydrogels are especially suited to hyperpolarize deuterated alcohols which can be used for in vivo perfusion imaging.
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Renal MR angiography and perfusion in the pig using hyperpolarized water
From The DNP-NMR Blog:
Renal MR angiography and perfusion in the pig using hyperpolarized water
Wigh Lipso, K., et al., Renal MR angiography and perfusion in the pig using hyperpolarized water. Magn Reson Med, 2016: p. n/a-n/a.
https://www.ncbi.nlm.nih.gov/pubmed/27690362
High-field ELDOR-detected NMR study of a nitroxide radical in disordered solids: Towards characterization of heterogeneity of microenvironments in spin-labeled systems
From The DNP-NMR Blog:
High-field ELDOR-detected NMR study of a nitroxide radical in disordered solids: Towards characterization of heterogeneity of microenvironments in spin-labeled systems
With nitroxides one of the most popular polarizing agents for DNP-NMR spectroscopy, this article gives more insights into the precise interactions (hyperfine, quadrupolar, g anisotropy ...) at high magnetic fields.
Nalepa, A., et al., High-field ELDOR-detected NMR study of a nitroxide radical in disordered solids: Towards characterization of heterogeneity of microenvironments in...
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08-04-2014 11:16 PM
[NMR paper] High-field ELDOR-detected NMR study of a nitroxide radical in disordered solids: Towards characterization of heterogeneity of microenvironments in spin-labeled systems.
High-field ELDOR-detected NMR study of a nitroxide radical in disordered solids: Towards characterization of heterogeneity of microenvironments in spin-labeled systems.
High-field ELDOR-detected NMR study of a nitroxide radical in disordered solids: Towards characterization of heterogeneity of microenvironments in spin-labeled systems.
J Magn Reson. 2014 Mar 12;242C:203-213
Authors: Nalepa A, Möbius K, Lubitz W, Savitsky A
Abstract
The combination of high-field EPR with site-directed spin-labeling (SDSL) techniques employing...
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04-02-2014 11:54 PM
High-field ELDOR-detected NMR study of a nitroxide radical in disordered solids:Towards characterization of heterogeneity of microenvironments in spin-labeled systems
High-field ELDOR-detected NMR study of a nitroxide radical in disordered solids:Towards characterization of heterogeneity of microenvironments in spin-labeled systems
Publication date: Available online 12 March 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Anna Nalepa , Klaus Möbius , Wolfgang Lubitz , Anton Savitsky</br>
The combination of high-field EPR with site-directed spin-labeling (SDSL) techniques employing nitroxide radicals has turned out to be particularly powerful in probing the polarity and proticity characteristics of protein/matrix...
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03-12-2014 01:35 PM
In vivo magnetic resonance imaging of hyperpolarized silicon particles
From The DNP-NMR Blog:
In vivo magnetic resonance imaging of hyperpolarized silicon particles
Cassidy, M.C., et al., In vivo magnetic resonance imaging of hyperpolarized silicon particles. Nat Nano, 2013. 8(5): p. 363-368.
http://dx.doi.org/10.1038/nnano.2013.65
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08-29-2013 01:27 AM
Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging
From the The DNP-NMR Blog:
Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging
Park, J.M., et al., Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging. Neuro-Oncology, 2013. 15(4): p. 433-41.
http://neuro-oncology.oxfordjournals.org/content/early/2013/01/16/neuonc.nos319.abstract
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04-15-2013 08:52 AM
An economical method for producing stable-isotope labeled proteins by the E. coli cel
An economical method for producing stable-isotope labeled proteins by the E. coli cell-free system
Abstract Improvement of the cell-free protein synthesis system (CF) over the past decade have made it one of the most powerful protein production methods. The CF approach is especially useful for stable-isotope (SI) labeling of proteins for NMR analysis. However, it is less popular than expected, partly because the SI-labeled amino acids used for SI labeling by the CF are too expensive. In the present study, we developed a simple and inexpensive method for producing an SI-labeled protein...
Producing Radical-Free Hyperpolarized Perfusion Agents for In Vivo Magnetic Resonance Using Spin-Labeled Thermoresponsive Hydrogel
Linear Mode
