On The Potential of Dynamic Nuclear Polarization Enhanced Diamonds in Solid-State and Dissolution 13 C NMR Spectroscopy #DNPNMR

		BioNMR > NMR community > News from NMR blogs
On The Potential of Dynamic Nuclear Polarization Enhanced Diamonds in Solid-State and Dissolution 13 C NMR Spectroscopy #DNPNMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-26-2016, 06:56 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

On The Potential of Dynamic Nuclear Polarization Enhanced Diamonds in Solid-State and Dissolution 13 C NMR Spectroscopy #DNPNMR

From The DNP-NMR Blog:

On The Potential of Dynamic Nuclear Polarization Enhanced Diamonds in Solid-State and Dissolution 13 C NMR Spectroscopy #DNPNMR

Bretschneider, C.O., et al., On The Potential of Dynamic Nuclear Polarization Enhanced Diamonds in Solid-State and Dissolution 13 C NMR Spectroscopy. ChemPhysChem, 2016: p. n/a-n/a.

http://www.ncbi.nlm.nih.gov/pubmed/27416769

Dynamic nuclear polarization (DNP) is a versatile option to improve the sensitivity of NMR and MRI. This versatility has elicited interest for overcoming potential limitations of these techniques, including the achievement of solid-state polarization enhancement at ambient conditions, and the maximization of 13 C signal lifetimes for performing in vivo MRI scans. This study explores whether diamond's 13 C behavior in nano- and micro-particles could be used to achieve these ends. The characteristics of diamond's DNP enhancement were analyzed for different magnetic fields, grain sizes, and sample environments ranging from cryogenic to ambient temperatures, in both solution and solid-state experiments. It was found that 13 C NMR signals could be boosted by orders of magnitude in either low- or room-temperature solid-state DNP experiments by utilizing naturally occurring paramagnetic P1 substitutional nitrogen defects. We attribute this behavior to the unusually long electronic/nuclear spin-lattice relaxation times characteristic of diamond, coupled with a time-independent cross-effect-like polarization transfer mechanism facilitated by a matching of the nitrogen-related hyperfine coupling and the 13 C Zeeman splitting. The efficiency of this solid-state polarization process, however, is harder to exploit in dissolution DNP-enhanced MRI contexts. The prospects for utilizing polarized diamond approaching nanoscale dimensions for both solid and solution applications are briefly discussed.

Go to The DNP-NMR Blog for more info.

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies #DNPNMR From The DNP-NMR Blog: Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies #DNPNMR Gupta, R., et al., Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies. J Phys Chem B, 2016. 120(2): p. 329-39. http://www.ncbi.nlm.nih.gov/pubmed/26709853	nmrlearner	News from NMR blogs	0	05-25-2016 02:33 PM
Towards structure determination of self-assembled peptides using dynamic nuclear polarization enhanced solid-state NMR spectroscopy From The DNP-NMR Blog: Towards structure determination of self-assembled peptides using dynamic nuclear polarization enhanced solid-state NMR spectroscopy Takahashi, H., et al., Towards structure determination of self-assembled peptides using dynamic nuclear polarization enhanced solid-state NMR spectroscopy. Angew Chem Int Ed Engl, 2013. 52(27): p. 6979-82. http://www.ncbi.nlm.nih.gov/pubmed/23564735	nmrlearner	News from NMR blogs	0	04-23-2014 06:31 PM
[NMR paper] Dynamic Nuclear Polarization Enhanced NMR in the Solid-State. Dynamic Nuclear Polarization Enhanced NMR in the Solid-State. Dynamic Nuclear Polarization Enhanced NMR in the Solid-State. Top Curr Chem. 2013 Jul 7; Authors: Akbey U, Franks WT, Linden A, Rydmark MO, Lange S, Oschkinat H Abstract Nuclear magnetic resonance (NMR) spectroscopy is one of the most commonly used spectroscopic techniques to obtain information on the structure and dynamics of biological and chemical materials. A variety of samples can be studied including solutions, crystalline solids, powders and hydrated protein...	nmrlearner	Journal club	0	07-09-2013 02:47 PM
Towards Structure Determination of Self-Assembled Peptides Using Dynamic Nuclear Polarization Enhanced Solid-State NMR Spectroscopy From The DNP-NMR Blog: Towards Structure Determination of Self-Assembled Peptides Using Dynamic Nuclear Polarization Enhanced Solid-State NMR Spectroscopy Takahashi, H., et al., Towards Structure Determination of Self-Assembled Peptides Using Dynamic Nuclear Polarization Enhanced Solid-State NMR Spectroscopy. Angew. Chem. Int. Ed., 2013: p. n/a-n/a. http://www.ncbi.nlm.nih.gov/pubmed/23564735	nmrlearner	News from NMR blogs	0	06-13-2013 04:24 AM
Enhanced Solid-State NMR Correlation Spectroscopy of Quadrupolar Nuclei Using Dynamic Nuclear Polarization Enhanced Solid-State NMR Correlation Spectroscopy of Quadrupolar Nuclei Using Dynamic Nuclear Polarization Daniel Lee, Hiroki Takahashi, Aany S. L. Thankamony, Jean-Philippe Dacquin, Michel Bardet, Olivier Lafon and Gae?l De Pae?pe http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja307755t/aop/images/medium/ja-2012-07755t_0004.gif Journal of the American Chemical Society DOI: 10.1021/ja307755t http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/NcXbDBD9aBc	nmrlearner	Journal club	0	11-03-2012 03:56 PM
Chemical Shifts for the Unusual DNA Structure in Pf1 Bacteriophage from Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR Spectroscopy Chemical Shifts for the Unusual DNA Structure in Pf1 Bacteriophage from Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR Spectroscopy Ivan V. Sergeyev, Loren A. Day, Amir Goldbourt and Ann E. McDermott http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja2043062/aop/images/medium/ja-2011-043062_0007.gif Journal of the American Chemical Society DOI: 10.1021/ja2043062 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/EeKgo5vg1K0	nmrlearner	Journal club	0	11-30-2011 10:45 PM
Chemical Shifts for the Unusual DNA Structure in Pf1 Bacteriophage from Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR Spectroscopy. Chemical Shifts for the Unusual DNA Structure in Pf1 Bacteriophage from Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR Spectroscopy. Chemical Shifts for the Unusual DNA Structure in Pf1 Bacteriophage from Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR Spectroscopy. J Am Chem Soc. 2011 Aug 22; Authors: Sergeyev IV, Day LA, Goldbourt A, McDermott AE Abstract Solid state NMR spectra, including dynamic nuclear polarization enhanced 400 MHz spectra acquired at 100K, as well as non-DNP spectra at a variety of field strengths and...	nmrlearner	Journal club	0	08-23-2011 04:03 PM
Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocry Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocrystals and amyloid fibrils. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocrystals and amyloid fibrils. Phys Chem Chem Phys. 2010 Jun 14;12(22):5911-9 Authors: Debelouchina GT, Bayro MJ, van der Wel PC, Caporini MA, Barnes AB, Rosay M, Maas WE, Griffin RG Dynamic nuclear polarization (DNP) utilizes the...	nmrlearner	Journal club	0	08-26-2010 04:41 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off