Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks
Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks
Abstract 1H NMR metabolic profiling of urine, serum and plasma has been used to monitor the impact of the pre-analytical steps on the sample quality and stability in order to propose standard operating procedures (SOPs) for deposition in biobanks. We analyzed the quality of serum and plasma samples as a function of the elapsed time (t = 0â??4 h) between blood collection and processing and of the time from processing to freezing (up to 24 h). The stability of the urine...
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[NMR paper] Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme com
Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Use of a truncated protein domain in NMR spectroscopy.
Related Articles Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Use of a truncated protein domain in NMR spectroscopy.
FEBS J. 2005 Jan;272(1):259-68
Authors: Allen MD, Broadhurst RW, Solomon RG, Perham RN
A (15)N-labelled peripheral-subunit binding domain (PSBD) of the dihydrolipoyl...
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11-24-2010 11:14 PM
[NMR paper] Solution 1H NMR of the molecular and electronic structure of the heme cavity and subs
Solution 1H NMR of the molecular and electronic structure of the heme cavity and substrate binding pocket of high-spin ferric horseradish peroxidase: effect of His42Ala mutation.
Related Articles Solution 1H NMR of the molecular and electronic structure of the heme cavity and substrate binding pocket of high-spin ferric horseradish peroxidase: effect of His42Ala mutation.
J Am Chem Soc. 2001 May 9;123(18):4243-54
Authors: Asokan A, de Ropp JS, Newmyer SL, Ortiz de Montellano PR, La Mar GN
Solution 1H NMR has been used to assign a major portion...
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11-19-2010 08:32 PM
[NMR paper] Principal components analysis of protein structure ensembles calculated using NMR dat
Principal components analysis of protein structure ensembles calculated using NMR data.
Related Articles Principal components analysis of protein structure ensembles calculated using NMR data.
J Biomol NMR. 2001 May;20(1):61-70
Authors: Howe PW
One important problem when calculating structures of biomolecules from NMR data is distinguishing converged structures from outlier structures. This paper describes how Principal Components Analysis (PCA) has the potential to classify calculated structures automatically, according to correlated...
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[NMR paper] 19F NMR study of protein-induced rhombic perturbations on the electronic structure of
19F NMR study of protein-induced rhombic perturbations on the electronic structure of the active site of myoglobin.
Related Articles 19F NMR study of protein-induced rhombic perturbations on the electronic structure of the active site of myoglobin.
J Biol Inorg Chem. 2000 Aug;5(4):455-62
Authors: Yamamoto Y, Hirai Y, Suzuki A
A novel C2-symmetric ring-fluorinated hemin, 13,17-bis(2-carboxyethyl)-2,8,12,18-tetramethyl-3,7-difluoroporphyrin atoiron(III), has been synthesized and was incorporated into sperm whale apomyoglobin to investigate...
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[NMR paper] 1H NMR investigation of the electronic and molecular structure of the four-iron clust
1H NMR investigation of the electronic and molecular structure of the four-iron cluster ferredoxin from the hyperthermophile Pyrococcus furiosus. Identification of Asp 14 as a cluster ligand in each of the four redox states.
Related Articles 1H NMR investigation of the electronic and molecular structure of the four-iron cluster ferredoxin from the hyperthermophile Pyrococcus furiosus. Identification of Asp 14 as a cluster ligand in each of the four redox states.
Biochemistry. 1995 Sep 12;34(36):11373-84
Authors: Calzolai L, Gorst CM, Zhao ZH, Teng Q,...
[Stan NMR blog] Passive Electronic Components: Color Codes
Passive Electronic Components: Color Codes
An educational article on color coding of resistors, inductors and capacitors as defined by IEC 62.
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Passive Electronic Components: Standard Values
Linear Mode
