Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment
Combinatorial triple-selective labeling as a tool to assist membrane protein backbone resonance assignment
Abstract Obtaining NMR assignments for slowly tumbling molecules such as detergent-solubilized membrane proteins is often compromised by low sensitivity as well as spectral overlap. Both problems can be addressed by amino-acid specific isotope labeling in conjunction with 15Nā??1H correlation experiments. In this work an extended combinatorial selective in vitro labeling scheme is proposed that seeks to reduce the number of samples required for assignment. Including three...
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01-21-2012 06:26 PM
A simplified recipe for assigning amide NMR signals using combinatorial (14)N amino acid inverse-labeling.
A simplified recipe for assigning amide NMR signals using combinatorial (14)N amino acid inverse-labeling.
A simplified recipe for assigning amide NMR signals using combinatorial (14)N amino acid inverse-labeling.
J Struct Funct Genomics. 2011 Aug 25;
Authors: Hiroaki H, Umetsu Y, Nabeshima YI, Hoshi M, Kohda D
Abstract
Assignment of backbone amide proton resonances is one of the most time-consuming stages of any protein NMR study when the protein samples behave non-ideally. A robust and convenient NMR procedure for analyzing spectra of...
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08-26-2011 04:22 PM
[NMR paper] Effect of pH and copper(II) on the conformation transitions of silk fibroin based on
Effect of pH and copper(II) on the conformation transitions of silk fibroin based on EPR, NMR, and Raman spectroscopy.
Related Articles Effect of pH and copper(II) on the conformation transitions of silk fibroin based on EPR, NMR, and Raman spectroscopy.
Biochemistry. 2004 Sep 28;43(38):11932-41
Authors: Zong XH, Zhou P, Shao ZZ, Chen SM, Chen X, Hu BW, Deng F, Yao WH
Much attention has been paid to the natural mechanism of silkworm spinning due to the impressive mechanical properties of the natural fibers. Our results in the present work show...
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11-24-2010 10:01 PM
[NMR paper] A combinatorial selective labeling method for the assignment of backbone amide NMR re
A combinatorial selective labeling method for the assignment of backbone amide NMR resonances.
Related Articles A combinatorial selective labeling method for the assignment of backbone amide NMR resonances.
J Am Chem Soc. 2004 Apr 28;126(16):5020-1
Authors: Parker MJ, Aulton-Jones M, Hounslow AM, Craven CJ
A combinatorial selective labeling (CSL) method is presented for the assignment of backbone amide NMR resonances, which has a particular application in the identification of protein-ligand interaction sites. The method builds on the dual...
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11-24-2010 09:51 PM
[NMR paper] NMR evidence for mechanical coupling of phosphate B(I)-B(II) transitions with deoxyri
NMR evidence for mechanical coupling of phosphate B(I)-B(II) transitions with deoxyribose conformational exchange in DNA.
Related Articles NMR evidence for mechanical coupling of phosphate B(I)-B(II) transitions with deoxyribose conformational exchange in DNA.
J Mol Biol. 2001 Aug 3;311(1):149-60
Authors: Isaacs RJ, Spielmann HP
The conformational exchange of the phosphate and deoxyribose groups of the DNA oligomers d(GCGTACGC)(2) and d(CGCTAGCG)(2) have been investigated using a combination of homonuclear and heteronuclear NMR techniques....
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11-19-2010 08:44 PM
[NMR paper] An NMR spectroscopic identity test for the control of the capsular polysaccharide fro
An NMR spectroscopic identity test for the control of the capsular polysaccharide from Haemophilus influenzae type b.
Related Articles An NMR spectroscopic identity test for the control of the capsular polysaccharide from Haemophilus influenzae type b.
Biologicals. 2000 Sep;28(3):175-83
Authors: Lemercinier X, Jones C
We describe the use of Nuclear Magnetic Resonance (NMR) spectroscopy to control the identity of purified bulk capsular polysaccharide from Haemophilus influenzae type b (Hib), and derivatised forms, used in the production of Hib...
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11-19-2010 08:29 PM
[NMR paper] NMR spectroscopy in drug discovery: tools for combinatorial chemistry, natural produc
NMR spectroscopy in drug discovery: tools for combinatorial chemistry, natural products, and metabolism research.
Related Articles NMR spectroscopy in drug discovery: tools for combinatorial chemistry, natural products, and metabolism research.
Prog Drug Res. 2000;55:137-211
Authors: Keifer PA
NMR spectroscopy has enjoyed many advances recently, and the pace of development shows no signs of slowing. This article focuses on advances that have affected solution-state NMR. These advances fall into three general categories: new experimental...
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11-18-2010 09:15 PM
[NMR paper] Phospholipid phase transitions as revealed by NMR.
Phospholipid phase transitions as revealed by NMR.
Related Articles Phospholipid phase transitions as revealed by NMR.
Chem Phys Lipids. 1991 Mar;57(2-3):195-211
Authors: Watts A, Spooner PJ
Aqueous dispersions of phospholipids can adopt a range of polymorphic phases which include bilayer and non-bilayer forms. Within the bilayer form, laterally separated phases may be induced as a result of surface electrostatic associations, thermotropic behaviour, lipid-protein interactions or because of molecular mismatch between chemically distinct...
p-Quantum Transitions and a Combinatorial Identity
Linear Mode
