Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low viscosity fluids

		BioNMR > NMR community > News from NMR blogs
Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low viscosity fluids

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

05-23-2014, 03:21 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low viscosity fluids

From The DNP-NMR Blog:

Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low viscosity fluids

Recently the group of Joshua Wand has described the use of reverse micelles as a platform for DNP for solution-state NMR spectroscopy. Although this article is not directly related to DNP it gives more insight into the use of reverse micelles to obtain high resolution NMR spectra of large marcromolecules.

Dodevski, I., et al., Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low viscosity fluids. J Am Chem Soc, 2014. 136(9): p. 3465-74.

http://www.ncbi.nlm.nih.gov/pubmed/24495164

An optimized reverse micelle surfactant system has been developed for solution nuclear magnetic resonance studies of encapsulated proteins and nucleic acids dissolved in low viscosity fluids. Comprising the nonionic 1-decanoyl-rac-glycerol and the zwitterionic lauryldimethylamine-N-oxide (10MAG/LDAO), this mixture is shown to efficiently encapsulate a diverse set of proteins and nucleic acids. Chemical shift analyses of these systems show that high structural fidelity is achieved upon encapsulation. The 10MAG/LDAO surfactant system reduces the molecular reorientation time for encapsulated macromolecules larger than ~20 kDa leading to improved overall NMR performance. The 10MAG/LDAO system can also be used for solution NMR studies of lipid-modified proteins. New and efficient strategies for optimization of encapsulation conditions are described. 10MAG/LDAO performs well in both the low viscosity pentane and ultralow viscosity liquid ethane and therefore will serve as a general surfactant system for initiating solution NMR studies of proteins and nucleic acids.

Go to The DNP-NMR Blog for more info.

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] High-resolution NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids. High-resolution NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids. Related Articles High-resolution NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids. J Magn Reson. 2014 Apr;241:137-47 Authors: Nucci NV, Valentine KG, Wand AJ Abstract	nmrlearner	Journal club	0	03-25-2014 11:49 AM
High-resolution NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids High-resolution NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids Publication date: April 2014 Source:Journal of Magnetic Resonance, Volume 241</br> Author(s): Nathaniel V. Nucci , Kathleen G. Valentine , A. Joshua Wand</br> High-resolution multi-dimensional solution NMR is unique as a biophysical and biochemical tool in its ability to examine both the structure and dynamics of macromolecules at atomic resolution. Conventional solution NMR approaches, however, are largely limited to examinations of relatively small (<25kDa) molecules,...	nmrlearner	Journal club	0	03-21-2014 12:52 AM
OptimizedReverse Micelle Surfactant System for High-ResolutionNMR Spectroscopy of Encapsulated Proteins and Nucleic Acids Dissolvedin Low Viscosity Fluids OptimizedReverse Micelle Surfactant System for High-ResolutionNMR Spectroscopy of Encapsulated Proteins and Nucleic Acids Dissolvedin Low Viscosity Fluids Igor Dodevski, Nathaniel V. Nucci, Kathleen G. Valentine, Gurnimrat K. Sidhu, Evan S. O’Brien, Arthur Pardi and A. Joshua Wand http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja410716w/aop/images/medium/ja-2013-10716w_0009.gif Journal of the American Chemical Society DOI: 10.1021/ja410716w http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...	nmrlearner	Journal club	0	02-20-2014 05:15 AM
[NMR paper] Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low-viscosity fluids. Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low-viscosity fluids. Related Articles Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low-viscosity fluids. J Am Chem Soc. 2014 Feb 4; Authors: Dodevski I, Nucci NV, Valentine KG, Sidhu GK, O'Brien ES, Pardi A, Wand AJ Abstract An optimized reverse micelle surfactant system has been developed for solution nuclear...	nmrlearner	Journal club	0	02-06-2014 12:47 PM
Optimization of NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids Optimization of NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids Abstract Comprehensive application of solution NMR spectroscopy to studies of macromolecules remains fundamentally limited by the molecular rotational correlation time. For proteins, molecules larger than 30 kDa require complex experimental methods, such as TROSY in conjunction with isotopic labeling schemes that are often expensive and generally reduce the potential information available. We have developed the reverse micelle encapsulation strategy as an alternative approach. Encapsulation of...	nmrlearner	Journal club	0	07-15-2011 09:10 PM
Optimization of NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids. Optimization of NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids. Optimization of NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids. J Biomol NMR. 2011 Jul 12; Authors: Nucci NV, Marques BS, Bédard S, Dogan J, Gledhill JM, Moorman VR, Peterson RW, Valentine KG, Wand AL, Wand AJ Comprehensive application of solution NMR spectroscopy to studies of macromolecules remains fundamentally limited by the molecular rotational correlation time. For proteins, molecules larger than 30*kDa require complex...	nmrlearner	Journal club	0	07-13-2011 06:42 PM
[NMR paper] Novel surfactant mixtures for NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids. Novel surfactant mixtures for NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids. Related Articles Novel surfactant mixtures for NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids. Protein Sci. 2005 Nov;14(11):2919-21 Authors: Peterson RW, Pometun MS, Shi Z, Wand AJ NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids is emerging as a tool for biophysical studies of proteins in atomic detail in a variety of otherwise inaccessible contexts. The central element of the...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] High-resolution NMR of encapsulated proteins dissolved in low-viscosity fluids. High-resolution NMR of encapsulated proteins dissolved in low-viscosity fluids. Related Articles High-resolution NMR of encapsulated proteins dissolved in low-viscosity fluids. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15299-302 Authors: Wand AJ, Ehrhardt MR, Flynn PF The majority of known proteins are too large to be comprehensively examined by solution NMR methods, primarily because they tumble too slowly in solution. Here we introduce an approach to making the NMR relaxation properties of large proteins amenable to modern solution NMR...	nmrlearner	Journal club	0	11-17-2010 11:15 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off