I bought the starter kit (NE-373-B-6/4.2) at NewEraNMR for RDC experiment on Varian NMR (with 700 MHz grade) and I decided to stretch the gel a lot, so 6mm-wide gel chamber and “6mm-4.2mm gel funnel” are included. The NMR tube seems to be more fragile than Wilmad products I often used, so I also ordered [...]
A simplified recipe for assigning amide NMR signals using combinatorial (14)N amino acid inverse-labeling.
A simplified recipe for assigning amide NMR signals using combinatorial (14)N amino acid inverse-labeling.
A simplified recipe for assigning amide NMR signals using combinatorial (14)N amino acid inverse-labeling.
J Struct Funct Genomics. 2011 Aug 25;
Authors: Hiroaki H, Umetsu Y, Nabeshima YI, Hoshi M, Kohda D
Abstract
Assignment of backbone amide proton resonances is one of the most time-consuming stages of any protein NMR study when the protein samples behave non-ideally. A robust and convenient NMR procedure for analyzing spectra of...
nmrlearner
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08-26-2011 04:22 PM
[NMR paper] NMR data collection and analysis protocol for high-throughput protein structure determination.
NMR data collection and analysis protocol for high-throughput protein structure determination.
Related Articles NMR data collection and analysis protocol for high-throughput protein structure determination.
Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10487-92
Authors: Liu G, Shen Y, Atreya HS, Parish D, Shao Y, Sukumaran DK, Xiao R, Yee A, Lemak A, Bhattacharya A, Acton TA, Arrowsmith CH, Montelione GT, Szyperski T
A standardized protocol enabling rapid NMR data collection for high-quality protein structure determination is presented that...
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12-01-2010 06:56 PM
[NMR paper] Cell-free protein production and labeling protocol for NMR-based structural proteomic
Cell-free protein production and labeling protocol for NMR-based structural proteomics.
Related Articles Cell-free protein production and labeling protocol for NMR-based structural proteomics.
Nat Methods. 2004 Nov;1(2):149-53
Authors: Vinarov DA, Lytle BL, Peterson FC, Tyler EM, Volkman BF, Markley JL
Structural proteomics requires robust, scalable methods. Here we describe a wheat germ cell-free platform for protein production that supports efficient NMR structural studies of eukaryotic proteins and offers advantages over cell-based methods....
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11-24-2010 10:01 PM
[NMR paper] Sorting signals from protein NMR spectra: SPI, a Bayesian protocol for uncovering spi
Sorting signals from protein NMR spectra: SPI, a Bayesian protocol for uncovering spin systems.
Related Articles Sorting signals from protein NMR spectra: SPI, a Bayesian protocol for uncovering spin systems.
J Biomol NMR. 2002 Nov;24(3):203-13
Authors: Grishaev A, Llinás M
Grouping of spectral peaks into J-connected spin systems is essential in the analysis of macromolecular NMR data as it provides the basis for disentangling chemical shift degeneracies. It is a mandatory step before resonance and NOESY cross-peak identities can be...
nmrlearner
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11-24-2010 08:58 PM
How do I make ambiguous assignments in NMRView for Aria?
Hi,
I'm trying to learn NMRView.
If one has an overlapped peak (multiple possible assignments) and wishes to leave this peak as ambiguous for ARIA to decide on, how does one 'assign it' as ambiguous in NMRView?
I suspect either of the following:
1) Leave question marks in the assignment boxes (aka leave the peak unassigned aka do nothing)
John
NMR software
1
04-27-2007 08:25 PM
How much does a NMR technician usually make?
http://www.chem.unl.edu/nmr/400mhz.htmlwell our professor said it was around 8 k a month, but im just seeing if those figures are correctim guessin no one has any of these at their school?
NMR protocol (recipe)?make stretch gel for protein RDC measurement
Linear Mode
