D.M. Korzhnev, T.L. Religa, W.Banachewicz, A.R. Fersht, L.E. Kay, "A Transient and Low-Populated Protein-Folding Intermediate at Atomic Resolution", Science329 (2010) 1312-1316. http://dx.doi.org/10.1126/science.1191723
Abstract: "Proteins can sample conformational states that are critical for function but are seldom detected directly because of their low occupancies and short lifetimes. In this work, we used chemical shifts and bond-vector orientation constraints obtained from nuclear magnetic resonance relaxation dispersion spectroscopy, in concert with a chemical shift–based method for structure elucidation, to determine an atomic-resolution structure of an "invisible" folding intermediate of a small protein module: the FF domain. The structure reveals non-native elements preventing formation of the native conformation in the carboxyl-terminal part of the protein. This is consistent with the kinetics of folding in which a well-structured intermediate forms rapidly and then rearranges slowly to the native state. The approach introduces a general strategy for structure determination of low-populated and transiently formed protein states."
This research article by Lewis Kay (University of Toronto) and colleagues is also accompanied by the Science Perspective
[NMR900 blog] NMR paper in Nature
NMR paper in Nature
G. Bouvignies, P. Vallurupalli, D.F. Hansen, B.E. Correia, O. Lange, A. Bah, R.M. Vernon, F.W. Dahlquist, D. Baker & L.E. Kay, "Solution structure of a minor and transiently formed state of a T4 lysozyme mutant," Nature 477 (2011) 111–114. http://dx.doi.org/10.1038/nature10349https://blogger.googleusercontent.com/tracker/8663203727601106205-5646954509437191595?l=nmr900.blogspot.com
Read complete story on NMR900 blog
[NMR900 blog] NMR paper in Nature Structural & Molecular Biology
NMR paper in Nature Structural & Molecular Biology
L.A. Freiburger, O.M. Baettig, T. Sprules, A.M. Berghuis, K. Auclair, A.K. Mittermaier, "Competing allosteric mechanisms modulate substrate binding in a dimeric enzyme," Nature Structural & Molecular Biology (2011) online. http://dx.doi.org/10.1038/nsmb.1978 https://blogger.googleusercontent.com/tracker/8663203727601106205-794484937251380301?l=nmr900.blogspot.com
Read complete story on NMR900 blog
nmrlearner
News from NMR blogs
0
02-11-2011 08:31 AM
[NMR900 blog] NMR paper in Angewandte Chemie
NMR paper in Angewandte Chemie
L. Shi, I. Kawamura, K.-H. Jung, L.S. Brown, and V. Ladizhansky, "Conformation of a Seven-Helical Transmembrane Photosensor in the Lipid Environment," Angewandte Chemie International Edition (2011) online. http://dx.doi.org/10.1002/anie.201004422
https://blogger.googleusercontent.com/tracker/8663203727601106205-792265230054630157?l=nmr900.blogspot.com
Read complete story on NMR900 blog
nmrlearner
News from NMR blogs
0
12-31-2010 07:40 AM
[CNS Yahoo group] Call For Research Paper - IJSER
Call For Research Paper - IJSER
International Journal of Scientific and Engineering Research (IJSER)(ISSN 2229-5518) The International Journal of Scientific & Engineering Research is an open
More...
NMR paper in Nature
NMR paper in Nature
A.M. Ruschak, T.L. Religa, S. Breuer, S. Witt and L.E. Kay, "The proteasome antechamber maintains substrates in an unfolded state," Nature 467 (2010) 868–871. http://dx.doi.org/10.1038/nature09444
https://blogger.googleusercontent.com/tracker/8663203727601106205-6883737347430659051?l=nmr900.blogspot.com
Read complete story on NMR900 blog