Nanodiamonds are of interest as nontoxic substrates for targeted drug delivery and as highly biostable fluorescent markers for cellular tracking. Beyond optical techniques, however, options for noninvasive imaging of nanodiamonds in vivo are severely limited. Here, we demonstrate that the Overhauser effect, a proton-electron polarization transfer technique, can enable high-contrast magnetic resonance imaging (MRI) of nanodiamonds in water at room temperature and ultra-low magnetic field. The technique transfers spin polarization from paramagnetic impurities at nanodiamond surfaces to 1H spins in the surrounding water solution, creating MRI contrast on-demand. We examine the conditions required for maximum enhancement as well as the ultimate sensitivity of the technique. The ability to perform continuous in situ hyperpolarization via the Overhauser mechanism, in combination with the excellent in vivo stability of nanodiamond, raises the possibility of performing noninvasive in vivo tracking of nanodiamond over indefinitely long periods of time.
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Hyperpolarized Nanodiamond Surfaces #DNPNMR
From The DNP-NMR Blog:
Hyperpolarized Nanodiamond Surfaces #DNPNMR
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Rej, E., et al., Hyperpolarized Nanodiamond Surfaces. J Am Chem Soc, 2017. 139(1): p. 193-199.
https://www.ncbi.nlm.nih.gov/pubmed/28009158
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02-15-2017 03:40 PM
Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR
From The DNP-NMR Blog:
Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR
Olsen, G., et al., Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR. J Magn Reson, 2016. 264: p. 49-58.
http://www.ncbi.nlm.nih.gov/pubmed/26920830
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05-09-2016 04:04 PM
[NMR paper] In situ NMR measurement of macromolecule-bound metal ion concentrations.
In situ NMR measurement of macromolecule-bound metal ion concentrations.
In situ NMR measurement of macromolecule-bound metal ion concentrations.
J Biomol NMR. 2016 Apr 23;
Authors: Kozlyuk N, Sengupta S, Lupták A, Martin RW
Abstract
Many nucleic acids and proteins require divalent metal ions such as Mg(2+) and Ca(2+) for folding and function. The lipophilic alignment media frequently used as membrane mimetics also bind these divalent metals. Here we demonstrate the use of (31)P NMR spectrum of a metal ion chelator...
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04-25-2016 11:58 AM
In situ NMR measurement of macromolecule-bound metal ion concentrations
In situ NMR measurement of macromolecule-bound metal ion concentrations
Abstract
Many nucleic acids and proteins require divalent metal ions such as Mg2+ and Ca2+ for folding and function. The lipophilic alignment media frequently used as membrane mimetics also bind these divalent metals. Here we demonstrate the use of 31P NMR spectrum of a metal ion chelator (deoxycytidine diphosphate) to measure the bound and in situ for several biological model systems at relatively high divalent ion concentrations (1â??10Â*mM). This method represents a general...
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04-23-2016 09:24 PM
[NMR paper] Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR.
Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR.
Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR.
J Magn Reson. 2016 Mar;264:49-58
Authors: Olsen G, Markhasin E, Szekely O, Bretschneider C, Frydman L
Abstract
A recent study explored the use of hyperpolarized water, to enhance the sensitivity of nuclei in biomolecules thanks to rapid proton exchanges with labile amide backbone and sidechain groups. Further optimizations of this...
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02-28-2016 03:10 PM
[NMR paper] Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR
Optimizing water hyperpolarization and dissolution for sensitivity-enhanced 2D biomolecular NMR
Publication date: March 2016
Source:Journal of Magnetic Resonance, Volume 264</br>
Author(s): Greg Olsen, Evgeny Markhasin, Or Szekely, Christian Bretschneider, Lucio Frydman</br>
A recent study explored the use of hyperpolarized water, to enhance the sensitivity of nuclei in biomolecules thanks to rapid proton exchanges with labile amide backbone and sidechain groups. Further optimizations of this approach have now allowed us to achieve proton polarizations...
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02-24-2016 01:30 AM
Hyperpolarized Nanodiamond with Long Spin Relaxation Times
From The DNP-NMR Blog:
Hyperpolarized Nanodiamond with Long Spin Relaxation Times
Rej E, Gaebel T, Boele T, Waddington D, Reilly D. Hyperpolarized Nanodiamond with Long Spin Relaxation Times. ARXIV. 2015.
http://arxiv.org/abs/1502.06214
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05-28-2015 12:56 AM
In Situ and Ex Situ Low-Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization
From The DNP-NMR Blog:
In Situ and Ex Situ Low-Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization
Barskiy, D.A., et al., In Situ and Ex Situ Low-Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization. ChemPhysChem, 2014. 15(18): p. 4100-7.
http://www.ncbi.nlm.nih.gov/pubmed/25367202
Nanodiamond-enhanced MRI via in situ hyperpolarization
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