[Question from NMRWiki Q&A forum] what is best pulse sequence for c13 edit noesy on vnmrj ?
what is best pulse sequence for c13 edit noesy on vnmrj ?
Hi
What is best pulse sequence in bio pack on vnmrj for C-13 edit NOESY ? I am working with 12 kd size protein which can exist as homo dimers ( i.e total size of homo-dimer is 24 kd ). Most frequent problems we are encountering as follows !line broadening , baseline , phase correction problems ( huge signal at 2.8 ppm )in first increments and f2f3 planes huge strips errors !
Thanking you in advance !
nmrlearner
News from other NMR forums
0
01-10-2012 03:38 PM
[Question from NMRWiki Q&A forum] CT COSY pulse sequence programming
CT COSY pulse sequence programming
Hi,
I want to make a CT-COSY pulse sequence. But I'm bother by incremented delay. When I type in the BaseLevelRelation.h this:
IN=-INand add in the microprogram the delay before and after my pi pulse,it didn't work... It seems like it calculate the d28 delay from the IN28 increment, but doesn't want to apply this delay in the pulse sequence....Any idea ? Perhaps it's about some incrementation declaration or something like that ?
nmrlearner
News from other NMR forums
0
05-04-2011 05:33 PM
[NMR analysis blog] Micropost: Pulse Sequence Blues!
Micropost: Pulse Sequence Blues!
Now that ENC is practically around the corner, here is a link that shows how far this conference encourages the participants’ creativity:
Pulse Sequence Blues!
NMR can also be fun :-)
https://blogger.googleusercontent.com/tracker/6953549091784501422-8328472542836523761?l=nmr-analysis.blogspot.com
nmrlearner
News from NMR blogs
0
02-23-2011 11:18 PM
[NMR paper] A sequential HNCA NMR pulse sequence for protein backbone assignment.
A sequential HNCA NMR pulse sequence for protein backbone assignment.
Related Articles A sequential HNCA NMR pulse sequence for protein backbone assignment.
J Magn Reson. 2001 May;150(1):100-4
Authors: Meissner A, Sørensen OW
The conventional HNCA pulse sequence suffers from the ambiguity that it cannot distinguish inter- and intraresidue correlations because the one-bond and two-bond J(NC(alpha)) coupling constants are of similar magnitude. This paper presents a novel pulse sequence, sequential HNCA, that leads to a spectrum exhibiting...
nmrlearner
Journal club
0
11-19-2010 08:32 PM
[NMR paper] A novel composite 90 degrees pulse sequence which provides distortionless NMR spectra
A novel composite 90 degrees pulse sequence which provides distortionless NMR spectra and suppresses without destroying the water magnetization.
Related Articles A novel composite 90 degrees pulse sequence which provides distortionless NMR spectra and suppresses without destroying the water magnetization.
J Magn Reson. 1998 Jul;133(1):194-9
Authors: Sodano P, Landon C, Ptak M
A novel 90 degrees composite pulse sequence which allows one to record 1D and 2D NMR spectra without disturbing the water magnetization is described. A home-written...
nmrlearner
Journal club
0
11-17-2010 11:15 PM
[Question from NMRWiki Q&A forum] Best pulse sequence for HSQC in Guanidine-HCl
Best pulse sequence for HSQC in Guanidine-HCl
Hello,
I would like to acquire a series of HSQC spectra of an 15N-labeled 12 kDa protein unfolding (over the course of hours) in 3M GuaHCl. I have access to a Varian 600 spectrometer with a conventional probe. Due to the high ionic strength of the sample, tuning and shimming is a little difficult. However, I can record a sort-of-decent HSQC using a WaterGate gradient enhanced WGgNhsqc sequence. I am limited to using a protein concentration of about 250 uM. I've determined the pw90 to be 12.125 at a tpwr of 63.
Using WGgNhsqc, the protein...
nmrlearner
News from other NMR forums
0
10-23-2010 07:42 AM
[NMRwiki tweet] nmrwiki: how to write #nmr pulse sequence with different carrier freqs in F1 and F3 ?
nmrwiki: how to write #nmr pulse sequence with different carrier freqs in F1 and F3 ? http://qa.nmrwiki.org/question/159/
nmrwiki: how to write #nmr pulse sequence with different carrier freqs in F1 and F3 ? http://qa.nmrwiki.org/question/159/
Source: NMRWiki tweets
nmrlearner
Twitter NMR
0
08-22-2010 01:49 AM
[NMRwiki tweet] nmrwiki: Download HZQC #NMR pulse sequence for Chemical Exchange http://nmrwiki.org/w
nmrwiki: Download HZQC #NMR pulse sequence for Chemical Exchange http://nmrwiki.org/wiki/index.php?title=HZQC/HDQC_for_Bruker_(hdqc_Zex.rp)
nmrwiki: Download HZQC #NMR pulse sequence for Chemical Exchange http://nmrwiki.org/wiki/index.php?title=HZQC/HDQC_for_Bruker_(hdqc_Zex.rp)
Source: NMRWiki tweets
Micropost: Pulse Sequence Blues!
Linear Mode
