Dynamic nuclear polarization (DNP) magic-angle spinning (MAS) solid-state NMR (ssNMR) spectroscopy has the potential to enhance NMR signals by orders of magnitude and to enable NMR characterization of proteins which are inherently dilute, such as membrane proteins. In this work spin-labeled lipid molecules (SL-lipids), when used as polarizing agents, lead to large and relatively homogeneous DNP enhancements throughout the lipid bilayer and to an embedded lung surfactant mimetic peptide, KL4 . Specifically, DNP MAS ssNMR experiments at 600 MHz/395 GHz on KL4 reconstituted in liposomes containing SL-lipids reveal DNP enhancement values over two times larger for KL4 compared to liposome suspensions containing the biradical TOTAPOL. These findings suggest an alternative sample preparation strategy for DNP MAS ssNMR studies of lipid membranes and integral membrane proteins.
Dynamic nuclear polarization enhancement of protons and vanadium-51 in the presence of pH-dependent vanadyl radicals
From The DNP-NMR Blog:
Dynamic nuclear polarization enhancement of protons and vanadium-51 in the presence of pH-dependent vanadyl radicals
Perez Linde, A.J., et al., Dynamic nuclear polarization enhancement of protons and vanadium-51 in the presence of pH-dependent vanadyl radicals. Magn Reson Chem, 2015. 53(2): p. 88-92.
http://www.ncbi.nlm.nih.gov/pubmed/25228149
nmrlearner
News from NMR blogs
0
02-27-2015 11:25 PM
[NMR paper] Solid-state NMR on bacterial cells: selective cell wall signal enhancement and resolution improvement using dynamic nuclear polarization.
Solid-state NMR on bacterial cells: selective cell wall signal enhancement and resolution improvement using dynamic nuclear polarization.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Solid-state NMR on bacterial cells: selective cell wall signal enhancement and resolution improvement using dynamic nuclear polarization.
J Am Chem Soc. 2013 Apr 3;135(13):5105-10
Authors: Takahashi H, Ayala I, Bardet M, De Paëpe G, Simorre JP, Hediger S
Abstract
...
nmrlearner
Journal club
0
10-14-2014 09:48 PM
Direct Enhancement of Nuclear Singlet Order by Dynamic Nuclear Polarization
From The DNP-NMR Blog:
Direct Enhancement of Nuclear Singlet Order by Dynamic Nuclear Polarization
I don't think I posted this one already.
Tayler, M.C.D., et al., Direct Enhancement of Nuclear Singlet Order by Dynamic Nuclear Polarization. J. Am. Chem. Soc., 2012. 134(18): p. 7668-7671.
nmrlearner
News from NMR blogs
0
02-27-2014 03:20 AM
Dynamic nuclear polarization methods in solids and solutions to explore membrane proteins and membrane systems
From The DNP-NMR Blog:
Dynamic nuclear polarization methods in solids and solutions to explore membrane proteins and membrane systems
Cheng, C.Y. and S. Han, Dynamic nuclear polarization methods in solids and solutions to explore membrane proteins and membrane systems. Annu Rev Phys Chem, 2013. 64(1): p. 507-32.
http://www.ncbi.nlm.nih.gov/pubmed/23331309
nmrlearner
News from NMR blogs
0
07-08-2013 02:17 PM
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
From The DNP-NMR Blog:
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
Kobayashi, T., et al., Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials. Phys Chem Chem Phys, 2013. 15(15): p. 5553-62.
http://www.ncbi.nlm.nih.gov/pubmed/23459985
nmrlearner
News from NMR blogs
0
04-17-2013 08:15 PM
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
From the The DNP-NMR Blog:
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
Kobayashi, T., et al., Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials. Phys Chem Chem Phys, 2013. 15(15): p. 5553-62.
http://www.ncbi.nlm.nih.gov/pubmed/23459985
nmrlearner
News from NMR blogs
0
04-15-2013 08:52 AM
Solid-State NMR on Bacterial Cells: Selective Cell Wall Signal Enhancement and Resolution Improvement using Dynamic Nuclear Polarization
From the The DNP-NMR Blog:
Solid-State NMR on Bacterial Cells: Selective Cell Wall Signal Enhancement and Resolution Improvement using Dynamic Nuclear Polarization
Takahashi, H., et al., Solid-State NMR on Bacterial Cells: Selective Cell Wall Signal Enhancement and Resolution Improvement using Dynamic Nuclear Polarization. J. Am. Chem. Soc., 2013.
http://dx.doi.org/10.1021/ja312501d
Dynamic nuclear polarization (DNP) enhanced solid-state nuclear magnetic resonance (NMR) has recently emerged as a powerful technique for the study of material surfaces. In this study, we demonstrate...
A method for dynamic nuclear polarization enhancement of membrane proteins
Linear Mode
