Fernandez-de-Alba, C., et al., Matrix-free DNP-enhanced NMR spectroscopy of liposomes using a lipid-anchored biradical. Chemistry, 2015. 21(12): p. 4512-7.
Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced.
[NMR paper] Dynamic nuclear polarization NMR spectroscopy: revealing multiple conformations in lipid-anchored peptide vaccines.
Dynamic nuclear polarization NMR spectroscopy: revealing multiple conformations in lipid-anchored peptide vaccines.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary_FullTextOnline_120x27.gif Related Articles Dynamic nuclear polarization NMR spectroscopy: revealing multiple conformations in lipid-anchored peptide vaccines.
Angew Chem Int Ed Engl. 2013 Oct 4;52(41):10905-8
Authors: Koers EJ, López-Deber MP, Weingarth M, Nand D, Hickman DT, Mlaki Ndao D, Reis P, Granet A, Pfeifer...
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04-24-2015 08:51 PM
DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature
From The DNP-NMR Blog:
DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature
Jakdetchai, O., et al., DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature. J Am Chem Soc, 2014.
http://www.ncbi.nlm.nih.gov/pubmed/25333422
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10-24-2014 07:18 PM
[NMR paper] DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature.
DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature.
Related Articles DNP-enhanced NMR on aligned Lipid Bilayers at ambient Temperature.
J Am Chem Soc. 2014 Oct 21;
Authors: Jakdetchai O, Denysenkov V, Becker-Baldus J, Dutagaci B, Prisner T, Glaubitz C
Abstract
DNP-enhanced solid-state NMR has been shown to hold great potential for functional studies of membrane proteins at low temperatures due to its great sensitivity improvement. There are however numerous applications, for which experiments at ambient temperature...
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10-22-2014 12:34 PM
Dynamic Nuclear Polarization NMR Spectroscopy: Revealing Multiple Conformations in Lipid-Anchored Peptide Vaccines
From The DNP-NMR Blog:
Dynamic Nuclear Polarization NMR Spectroscopy: Revealing Multiple Conformations in Lipid-Anchored Peptide Vaccines
Koers, E.J., et al., Dynamic Nuclear Polarization NMR Spectroscopy: Revealing Multiple Conformations in Lipid-Anchored Peptide Vaccines. Angew Chem Int Ed Engl, 2013. 52(41): p. 10905-10908.
http://www.ncbi.nlm.nih.gov/pubmed/24039018
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04-11-2014 02:51 PM
[NMR paper] Solid-state NMR Spectra of lipid-anchored Proteins under Magic Angle Spinning.
Solid-state NMR Spectra of lipid-anchored Proteins under Magic Angle Spinning.
Related Articles Solid-state NMR Spectra of lipid-anchored Proteins under Magic Angle Spinning.
J Phys Chem B. 2014 Feb 11;
Authors: Nomura K, Harada E, Sugase K, Shimamoto K
Abstract
Solid-state NMR is a promising tool for elucidating membrane-related biological phenomena. We achieved the measurement of high-resolution solid-state NMR spectra for a lipid-anchored protein embedded in lipid bilayers under magic angle spinning (MAS). To date, solid-state NMR...
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02-13-2014 03:35 PM
Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments
From The DNP-NMR Blog:
Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments
Takahashi, H., et al., Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments. J Magn Reson, 2013. 239C(0): p. 91-99.
http://www.ncbi.nlm.nih.gov/pubmed/24480716
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02-10-2014 08:46 PM
Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance
From The DNP-NMR Blog:
Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance
Takahashi, H., S. Hediger, and G. De Paepe, Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance. Chem Commun (Camb), 2013. 49(82): p. 9479-81.
http://www.ncbi.nlm.nih.gov/pubmed/24013616
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11-21-2013 01:14 AM
[NMR paper] Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance.
Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance.
Matrix-free dynamic nuclear polarization enables solid-state NMR (13)C-(13)C correlation spectroscopy of proteins at natural isotopic abundance.
Chem Commun (Camb). 2013 Sep 6;
Authors: Takahashi H, Hediger S, De Paëpe G
Abstract
We introduce a general approach for dynamic nuclear polarization (DNP) enhanced solid-state NMR that overcomes the current problems in DNP experiments caused by the use...
Matrix-free DNP-enhanced NMR spectroscopy of liposomes using a lipid-anchored biradical
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