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Lipsø, K.W., et al., Large dose hyperpolarized water with dissolution-DNP at high magnetic field. J. Magn. Reson., 2017. 274: p. 65-72.
We demonstrate a method for the preparation of hyperpolarized water by dissolution Dynamic Nuclear Polarization at high magnetic field. Protons were polarized at 6.7 T and 1.1 K to >70% with frequency modulated microwave irradiation at 188G Hz. 97.2 ± 0.7% of the radical was extracted from the sample in the dissolution in a two-phase system. 16 ± 1 mL of 5.0 M 1H in D2O with a polarization of 13.0 ± 0.9% in the liquid state was obtained, corresponding to an enhancement factor of 4000 ± 300 compared to the thermal equilibrium at 9.4 T and 293 K. A longitudinal relaxation time constant of 16 ± 1 s was measured. The sample was polarized and dissolved in a fluid path compatible with clinical polarizers. The volume of hyperpolarized water produced by this method enables angiography and perfusion measurements in large animals, as well as NMR experiments for studies of e.g. proton exchange and polarization transfer to other nuclei.
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[NMR paper] High quality NMR structures: a new force field with implicit water and membrane solvation for Xplor-NIH.
High quality NMR structures: a new force field with implicit water and membrane solvation for Xplor-NIH.
High quality NMR structures: a new force field with implicit water and membrane solvation for Xplor-NIH.
J Biomol NMR. 2016 Dec 29;:
Authors: Tian Y, Schwieters CD, Opella SJ, Marassi FM
Abstract
Structure determination of proteins by NMR is unique in its ability to measure restraints, very accurately, in environments and under conditions that closely mimic those encountered in vivo. For example, advances in solid-state NMR...
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12-31-2016 12:18 PM
High quality NMR structures: a new force field with implicit water and membrane solvation for Xplor-NIH
High quality NMR structures: a new force field with implicit water and membrane solvation for Xplor-NIH
Abstract
Structure determination of proteins by NMR is unique in its ability to measure restraints, very accurately, in environments and under conditions that closely mimic those encountered in vivo. For example, advances in solid-state NMR methods enable structure determination of membrane proteins in detergent-free lipid bilayers, and of large soluble proteins prepared by sedimentation, while parallel advances in solution NMR methods and...
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12-29-2016 09:23 PM
Milli-tesla NMR and spectrophotometry of liquids hyperpolarized by dissolution dynamic nuclear polarization
From The DNP-NMR Blog:
Milli-tesla NMR and spectrophotometry of liquids hyperpolarized by dissolution dynamic nuclear polarization
Zhu, Y., et al., Milli-tesla NMR and spectrophotometry of liquids hyperpolarized by dissolution dynamic nuclear polarization. J Magn Reson, 2016. 270: p. 71-6.
https://www.ncbi.nlm.nih.gov/pubmed/27423094
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09-26-2016 04:55 PM
High-field dissolution dynamic nuclear polarization of [1-(13)C]pyruvic acid
From The DNP-NMR Blog:
High-field dissolution dynamic nuclear polarization of pyruvic acid
Yoshihara, H.A., et al., High-field dissolution dynamic nuclear polarization of pyruvic acid. Phys Chem Chem Phys, 2016. 18(18): p. 12409-13.
http://www.ncbi.nlm.nih.gov/pubmed/27093499
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07-11-2016 04:06 PM
Enhanced performance large volume dissolution-DNP
From The DNP-NMR Blog:
Enhanced performance large volume dissolution-DNP
Bowen, S. and J.H. Ardenkjaer-Larsen, Enhanced performance large volume dissolution-DNP. J Magn Reson, 2014. 240(0): p. 90-4.
http://www.ncbi.nlm.nih.gov/pubmed/24531395
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05-22-2014 01:48 AM
High magnetic field science and its application in the United States: A magnetic resonance perspective
From The DNP-NMR Blog:
High magnetic field science and its application in the United States: A magnetic resonance perspective
Frydman, L., High magnetic field science and its application in the United States: A magnetic resonance perspective. J Magn Reson, 2014(0).
http://www.ncbi.nlm.nih.gov/pubmed/24618413
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04-28-2014 10:34 PM
High resolution NMR spectroscopy of nanocrystalline proteins at ultra-high magnetic field
High resolution NMR spectroscopy of nanocrystalline proteins at ultra-high magnetic field
Abstract Magic-angle spinning (MAS) solid-state NMR (SSNMR) spectroscopy of uniformly-13C,15N labeled protein samples provides insight into atomic-resolution chemistry and structure. Data collection efficiency has advanced remarkably in the last decade; however, the study of larger proteins is still challenged by relatively low resolution in comparison to solution NMR. In this study, we present a systematic analysis of SSNMR protein spectra acquired at 11.7, 17.6 and 21.1 Tesla (1H frequencies of...
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01-09-2011 12:46 PM
[NMR paper] Structure of the metal-water complex in Ras x GDP studied by high-field EPR spectrosc
Structure of the metal-water complex in Ras x GDP studied by high-field EPR spectroscopy and 31P NMR spectroscopy.
Related Articles Structure of the metal-water complex in Ras x GDP studied by high-field EPR spectroscopy and 31P NMR spectroscopy.
Biochemistry. 2001 Feb 20;40(7):1884-9
Authors: Rohrer M, Prisner TF, Brügmann O, Käss H, Spoerner M, Wittinghofer A, Kalbitzer HR
The small GTPase Ras plays a key role as a molecular switch in the intercellular signal transduction. On Mg(2+) --> Mn(2+) substituted samples, the first ligand sphere of...
Large dose hyperpolarized water with dissolution-DNP at high magnetic field
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