Instrumentation for solid-state dynamic nuclear polarization with magic angle spinning NMR

		BioNMR > NMR community > News from NMR blogs
Instrumentation for solid-state dynamic nuclear polarization with magic angle spinning NMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-18-2016, 03:17 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Instrumentation for solid-state dynamic nuclear polarization with magic angle spinning NMR

From The DNP-NMR Blog:

Instrumentation for solid-state dynamic nuclear polarization with magic angle spinning NMR

Rosay, M., M. Blank, and F. Engelke, Instrumentation for solid-state dynamic nuclear polarization with magic angle spinning NMR. J Magn Reson, 2016. 264: p. 88-98.

http://www.ncbi.nlm.nih.gov/pubmed/26920834

Advances in dynamic nuclear polarization (DNP) instrumentation and methodology have been key factors in the recent growth of solid-state DNP NMR applications. We review the current state of the art of solid-state DNP NMR instrumentation primarily based on available commercial platforms. We start with a general system overview, including options for microwave sources and DNP NMR probes, and then focus on specific developments for DNP at 100K with magic angle spinning (MAS). Gyrotron microwave sources, passive components to transmit microwaves, the DNP MAS probe, a cooling device for low-temperature MAS, and sample preparation procedures including radicals for DNP are considered.

Go to The DNP-NMR Blog for more info.

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Nuclear Depolarization and Absolute Sensitivity in Magic-Angle Spinning Cross-Effect Dynamic Nuclear Polarization From The DNP-NMR Blog: Nuclear Depolarization and Absolute Sensitivity in Magic-Angle Spinning Cross-Effect Dynamic Nuclear Polarization Mentink-Vigier, F., et al., Nuclear Depolarization and Absolute Sensitivity in Magic-Angle Spinning Cross-Effect Dynamic Nuclear Polarization. Phys. Chem. Chem. Phys., 2015. http://dx.doi.org/10.1039/C5CP03457D	nmrlearner	News from NMR blogs	0	07-30-2015 12:01 AM
Theoretical Aspects of Magic Angle Spinning - Dynamic Nuclear Polarization From The DNP-NMR Blog: Theoretical Aspects of Magic Angle Spinning - Dynamic Nuclear Polarization Mentink-Vigier, F., et al., Theoretical Aspects of Magic Angle Spinning - Dynamic Nuclear Polarization. J. Magn. Reson., 2015. http://www.sciencedirect.com/science/article/pii/S1090780715001500	nmrlearner	News from NMR blogs	0	07-27-2015 10:41 PM
Magic Angle Spinning NMR of Proteins: High-Frequency Dynamic Nuclear Polarization and H Detection From The DNP-NMR Blog: Magic Angle Spinning NMR of Proteins: High-Frequency Dynamic Nuclear Polarization and H Detection Su, Y., L. Andreas, and R.G. Griffin, Magic Angle Spinning NMR of Proteins: High-Frequency Dynamic Nuclear Polarization and H Detection. Annu Rev Biochem, 2015. http://www.ncbi.nlm.nih.gov/pubmed/25839340	nmrlearner	News from NMR blogs	0	04-17-2015 08:49 PM
[NMR paper] Magic Angle Spinning NMR of Proteins: High-Frequency Dynamic Nuclear Polarization and (1)H Detection. Magic Angle Spinning NMR of Proteins: High-Frequency Dynamic Nuclear Polarization and (1)H Detection. Magic Angle Spinning NMR of Proteins: High-Frequency Dynamic Nuclear Polarization and (1)H Detection. Annu Rev Biochem. 2015 Mar 30; Authors: Su Y, Andreas L, Griffin RG Abstract Magic angle spinning (MAS) NMR studies of amyloid and membrane proteins and large macromolecular complexes are an important new approach to structural biology. However, the applicability of these experiments, which are based on (13)C- and...	nmrlearner	Journal club	0	04-04-2015 05:31 PM
[NMR paper] Magic-angle spinning solid-state multinuclear NMR on low-field instrumentation. Magic-angle spinning solid-state multinuclear NMR on low-field instrumentation. Related Articles Magic-angle spinning solid-state multinuclear NMR on low-field instrumentation. J Magn Reson. 2013 Nov 1;238C:20-25 Authors: Sørensen MK, Bakharev O, Jensen O, Jakobsen HJ, Skibsted J, Nielsen NC Abstract Mobile and cost-effective NMR spectroscopy exploiting low-field permanent magnets is a field of tremendous development with obvious applications for arrayed large scale analysis, field work, and industrial screening. So far such...	nmrlearner	Journal club	0	12-03-2013 12:49 PM
Solid state nuclear magnetic resonance with magic-angle spinning and dynamic nuclear polarization below 25K Solid state nuclear magnetic resonance with magic-angle spinning and dynamic nuclear polarization below 25K January 2013 Publication year: 2013 Source:Journal of Magnetic Resonance, Volume 226</br> </br> We describe an apparatus for solid state nuclear magnetic resonance (NMR) with dynamic nuclear polarization (DNP) and magic-angle spinning (MAS) at 20–25K and 9.4Tesla. The MAS NMR probe uses helium to cool the sample space and nitrogen gas for MAS drive and bearings, as described earlier , but also includes a corrugated waveguide for transmission of microwaves from...	nmrlearner	Journal club	0	12-15-2012 09:51 AM
Solid state nuclear magnetic resonance with magic-angle spinning and dynamic nuclear polarization below 25 K Solid state nuclear magnetic resonance with magic-angle spinning and dynamic nuclear polarization below 25 K Available online 20 November 2012 Publication year: 2012 Source:Journal of Magnetic Resonance</br> </br> We describe an apparatus for solid state nuclear magnetic resonance (NMR) with dynamic nuclear polarization (DNP) and magic-angle spinning (MAS) at 20-25 K and 9.4 Tesla. The MAS NMR probe uses helium to cool the sample space and nitrogen gas for MAS drive and bearings, as described earlier (Thurber et al., J. Magn. Reson. 2008) , but also includes a...	nmrlearner	Journal club	0	12-01-2012 06:10 PM
Solid state nuclear magnetic resonance with magic-angle spinning and dynamic nuclear polarization below 25 K Solid state nuclear magnetic resonance with magic-angle spinning and dynamic nuclear polarization below 25 K Publication year: 2012 Source:Journal of Magnetic Resonance</br> Kent R. Thurber, Alexey Potapov, Wai-Ming Yau, Robert Tycko</br> We describe an apparatus for solid state nuclear magnetic resonance (NMR) with dynamic nuclear polarization (DNP) and magic-angle spinning (MAS) at 20-25 K and 9.4 Tesla. The MAS NMR probe uses helium to cool the sample space and nitrogen gas for MAS drive and bearings, as described earlier (Thurber et al., J. Magn. Reson. 2008) ,...	nmrlearner	Journal club	0	11-21-2012 04:33 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off