Natural abundance (13)C NMR spectra of biological extracts are recorded in a single scan provided that the samples are hyperpolarized by dissolution dynamic nuclear polarization combined with cross polarization. Heteronuclear 2D correlation spectra of hyperpolarized breast cancer cell extracts can also be obtained in a single scan. Hyperpolarized NMR of extracts opens many perspectives for metabolomics.
Natural Abundance (17)O DNP Two-Dimensional and Surface-Enhanced NMR Spectroscopy
From The DNP-NMR Blog:
Natural Abundance (17)O DNP Two-Dimensional and Surface-Enhanced NMR Spectroscopy
Perras, F.A., T. Kobayashi, and M. Pruski, Natural Abundance (17)O DNP Two-Dimensional and Surface-Enhanced NMR Spectroscopy. J Am Chem Soc, 2015. 137(26): p. 8336-9.
http://www.ncbi.nlm.nih.gov/pubmed/26098846
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09-28-2015 06:31 PM
[NMR paper] Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
Anal Chem. 2015 Apr 7;87(7):3556-61
Authors: Arbogast LW, Brinson RG, Marino JP
Abstract
Monoclonal antibodies (mAbs) represent an important and rapidly growing class of biotherapeutics. Correct folding of a mAb is critical for drug efficacy, while misfolding...
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09-19-2015 02:53 PM
Natural Abundance 17O DNP Two-Dimensional and Surface-Enhanced NMR Spectroscopy
Natural Abundance 17O DNP Two-Dimensional and Surface-Enhanced NMR Spectroscopy
Fre?de?ric A. Perras, Takeshi Kobayashi and Marek Pruski
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.5b03905/20150629/images/medium/ja-2015-03905b_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.5b03905
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/2XY9E0YdGEg
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06-29-2015 07:21 PM
Journal Highlight: Coupling of a high-resolution monoamine oxidase-A inhibitor assay and HPLC–SPE–NMR for advanced bioactivity profiling of plant extracts
Journal Highlight: Coupling of a high-resolution monoamine oxidase-A inhibitor assay and HPLC–SPE–NMR for advanced bioactivity profiling of plant extracts
http://www.spectroscopynow.com/common/images/thumbnails/13d26a3428e.jpgA photometric microplate-based high-resolution monoamine oxidase A inhibitor assay was coupled with an HPLC–SPE–tube transfer NMR system for the bioactivity profiling of plant extracts in the search for new antidepressants.
Read the rest at Spectroscopynow.com
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General
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03-04-2013 03:07 PM
Journal Highlight: Quantitative NMR: An applicable method for quantitative analysis of medicinal plant extracts and herbal products
Journal Highlight: Quantitative NMR: An applicable method for quantitative analysis of medicinal plant extracts and herbal products
http://www.spectroscopynow.com/common/images/thumbnails/13abcca009b.jpgA quantitative NMR method has been reported for quantitative analysis of three medicinal plant extracts and their herbal products without the need of authentic standards.
Source: Spectroscopynow.com
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02-03-2013 08:49 AM
[NMR paper] 1H- and natural abundance 15N-NMR studies of a derivative of a rabies glycoprotein fr
1H- and natural abundance 15N-NMR studies of a derivative of a rabies glycoprotein fragment.
Related Articles 1H- and natural abundance 15N-NMR studies of a derivative of a rabies glycoprotein fragment.
Biopolymers. 1991 May;31(6):713-23
Authors: Molinari H, Consonni R, Pegna M, Zetta L, Neri P, Niccolai N, Bonci A, Lozzi L, Rustici M, Scarselli M
Using a combination of one- and two-dimensional methods, 1H- and 15N-nmr spectroscopy has been employed to perform the complete assignment and the structural determination of the immunogenic...
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08-21-2010 11:16 PM
[NMR paper] Assignment of the natural abundance 13C spectrum of proteins using 13C 1H-detected he
Assignment of the natural abundance 13C spectrum of proteins using 13C 1H-detected heteronuclear multiple-bond correlation NMR spectroscopy: structural information and stereospecific assignments from two- and three-bond carbon-hydrogen coupling constants.
Related Articles Assignment of the natural abundance 13C spectrum of proteins using 13C 1H-detected heteronuclear multiple-bond correlation NMR spectroscopy: structural information and stereospecific assignments from two- and three-bond carbon-hydrogen coupling constants.
Biochemistry. 1991 Oct 29;30(43):10457-66
...
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08-21-2010 11:12 PM
[NMR paper] Assignment of the natural abundance 13C spectrum of proteins using 13C 1H-detected he
Assignment of the natural abundance 13C spectrum of proteins using 13C 1H-detected heteronuclear multiple-bond correlation NMR spectroscopy: structural information and stereospecific assignments from two- and three-bond carbon-hydrogen coupling constants.
Related Articles Assignment of the natural abundance 13C spectrum of proteins using 13C 1H-detected heteronuclear multiple-bond correlation NMR spectroscopy: structural information and stereospecific assignments from two- and three-bond carbon-hydrogen coupling constants.
Biochemistry. 1991 Oct 29;30(43):10457-66
...
Hyperpolarized NMR of plant and cancer cell extracts at natural abundance
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