Butyrate, a short chain fatty acid, was studied as a novel hyperpolarized substrate for use in dynamic nuclear polarization enhanced magnetic resonance spectroscopy experiments, to define the pathways of short chain fatty acid and ketone body metabolism in real time. METHODS: Butyrate was polarized via the dynamic nuclear polarization process and subsequently dissolved to generate an injectable metabolic substrate. Metabolism was initially assessed in the isolated perfused rat heart, followed by evaluation in the in vivo rat heart. RESULTS: Hyperpolarized butyrate was generated with a polarization level of 7% and was shown to have a T1 relaxation time of 20 s. These physical characteristics were sufficient to enable assessment of multiple steps in its metabolism, with the ketone body acetoacetate and several tricarboxylic acid cycle intermediates observed both in vitro and in vivo. Metabolite to butyrate ratios of 0.1-0.4% and 0.5-2% were observed in vitro and in vivo respectively, similar to levels previously observed with hyperpolarized [2-13 C]pyruvate. CONCLUSIONS: In this study, butyrate has been demonstrated to be a suitable hyperpolarized substrate capable of revealing multi-step metabolism in dynamic nuclear polarization experiments and providing information on the metabolism of fatty acids not currently achievable with other hyperpolarized substrates. Magn Reson Med, 2013. (c) 2013 Wiley Periodicals, Inc.
Transmit-Only/Receive-Only Radiofrequency System for Hyperpolarized 13C MRS Cardiac Metabolism Studies in Pigs
From The DNP-NMR Blog:
Transmit-Only/Receive-Only Radiofrequency System for Hyperpolarized 13C MRS Cardiac Metabolism Studies in Pigs
Giovannetti, G., et al., Transmit-Only/Receive-Only Radiofrequency System for Hyperpolarized 13C MRS Cardiac Metabolism Studies in Pigs. Appl. Magn. Reson., 2013. 44(10): p. 1125-1138.
http://dx.doi.org/10.1007/s00723-013-0477-3
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11-21-2013 01:14 AM
Analysis of Cancer Metabolism by Imaging Hyperpolarized Nuclei: Prospects for Translation to Clinical Research
From The DNP-NMR Blog:
Analysis of Cancer Metabolism by Imaging Hyperpolarized Nuclei: Prospects for Translation to Clinical Research
Kurhanewicz, J., et al., Analysis of cancer metabolism by imaging hyperpolarized nuclei: prospects for translation to clinical research. Neoplasia, 2011. 13(2): p. 81-97.
http://www.ncbi.nlm.nih.gov/pubmed/21403835
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Design of a quadrature surface coil for hyperpolarized 13C MRS cardiac metabolism studies in pigs
From The DNP-NMR Blog:
Design of a quadrature surface coil for hyperpolarized 13C MRS cardiac metabolism studies in pigs
Due to ENC I'm a bit behind catching up with the literature ... This article below was just published in Concepts in Magnetic Resonance and shows the diverse applications of DNP.
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04-27-2013 01:19 AM
‘q-titration’ of long-chain and short-chain lipids differentiates between structured and mobile residues of membrane proteins studied in bicelles by solution NMR spectroscopy
‘q-titration’ of long-chain and short-chain lipids differentiates between structured and mobile residues of membrane proteins studied in bicelles by solution NMR spectroscopy
Publication year: 2011
Source: Journal of Magnetic Resonance, Available online 25 October 2011</br>
Woo Sung*Son, Sang Ho*Park, Henry J.*Nothnagel, George J.*Lu, Yan*Wang, ...</br>
‘q-titration’ refers to the systematic comparison of signal intensities in solution NMR spectra of uniformlyN labeled membrane proteins solubilized in micelles and isotropic bicelles as a function of the molar ratios (q) of the...
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10-26-2011 11:25 AM
[NMR paper] Interaction of chicken liver basic fatty acid-binding protein with fatty acids: a 13C
Interaction of chicken liver basic fatty acid-binding protein with fatty acids: a 13C NMR and fluorescence study.
Related Articles Interaction of chicken liver basic fatty acid-binding protein with fatty acids: a 13C NMR and fluorescence study.
Biochemistry. 2001 Oct 23;40(42):12604-11
Authors: Beringhelli T, Goldoni L, Capaldi S, Bossi A, Perduca M, Monaco HL
Two different groups of liver fatty acid-binding proteins (L-FABPs) are known: the mammalian type and the basic type. Very few members of this second group of L-FABPs have been...
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11-19-2010 08:44 PM
[NMR paper] Bound water in apo and holo bovine heart fatty-acid-binding protein determined by het
Bound water in apo and holo bovine heart fatty-acid-binding protein determined by heteronuclear NMR spectroscopy.
Related Articles Bound water in apo and holo bovine heart fatty-acid-binding protein determined by heteronuclear NMR spectroscopy.
Eur J Biochem. 1998 Feb 1;251(3):781-6
Authors: Mesgarzadeh A, Pfeiffer S, Engelke J, Lassen D, Rüterjans H
Two- and three-dimensional heteronuclear NMR experiments have been performed to identify internally bound water molecules in the solution structure of bovine heart fatty-acid-binding protein...
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11-17-2010 11:06 PM
[NMR paper] Three-dimensional structure of bovine heart fatty-acid-binding protein with bound pal
Three-dimensional structure of bovine heart fatty-acid-binding protein with bound palmitic acid, determined by multidimensional NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Three-dimensional structure of bovine heart fatty-acid-binding protein with bound palmitic acid, determined by multidimensional NMR spectroscopy.
Eur J Biochem. 1995 May 15;230(1):266-80
Authors: Lassen D, Lücke C, Kveder M, Mesgarzadeh A, Schmidt JM,...
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08-22-2010 03:41 AM
[NMR paper] 13C NMR studies of fatty acid-protein interactions: comparison of homologous fatty ac
13C NMR studies of fatty acid-protein interactions: comparison of homologous fatty acid-binding proteins produced in the intestinal epithelium.
Related Articles 13C NMR studies of fatty acid-protein interactions: comparison of homologous fatty acid-binding proteins produced in the intestinal epithelium.
Mol Cell Biochem. 1990 Oct 15-Nov 8;98(1-2):101-10
Authors: Cistola DP, Sacchettini JC, Gordon JI
A high-resolution, solution-state NMR method for characterizing and comparing the interactions between carboxyl 13C-enriched fatty acids (FA) and...