Hyperpolarization Methods and Applications in NMR

		BioNMR > NMR community > News from NMR blogs
Hyperpolarization Methods and Applications in NMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2013, 08:49 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Hyperpolarization Methods and Applications in NMR

From The DNP-NMR Blog:

Hyperpolarization Methods and Applications in NMR

Köckenberger, W. and J. Matysik, Hyperpolarization Methods and Applications in NMR, in Encyclopedia of Spectroscopy and Spectrometry (Second Edition), L. Editor-in-Chief: John, Editor. 2010, Academic Press: Oxford. p. 963-970.

http://dx.doi.org/10.1016/B978-0-12-374413-5.00054-3

Nuclear magnetic resonance (NMR) is a well known and versatile spectroscopic and analytical technique. Its enormous success is due to its capacity to determine structures of molecules and proteins in solutions and amorphous solids, to measure local mobilities, to provide information on reaction mechanisms, and to construct three-dimensional images. The main constraints of NMR are its limitation to microsecond time resolution and the low sensitivity. The latter problem is caused by the weak magnetic properties of nuclei and a resulting unfavorable Boltzmann distribution occurring under equilibrium conditions. Therefore, several methods have been developed to produce non-Boltzmann nuclear spin states leading to hyperpolarization and enhanced sensitivity. These hyperpolarization methods induce spin-chemical processes that overcome the Boltzmann equilibrium of the nuclear spins. Under these conditions, signals enhanced to a factor of more than 10 000 are observed. Hyperpolarization methods use light, microwaves, or chemical reactions and, therefore, link different forms of spectroscopy.

Go to The DNP-NMR Blog for more info.

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Long-Range Proton-Carbon Coupling Constants: NMR methods and applications Long-Range Proton-Carbon Coupling Constants: NMR methods and applications Publication date: Available online 20 July 2013 Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br> Author(s): Teodor Parella , Juan Félix Espinosa</br> A general review of novel NMR methods to measure heteronuclear long-range proton-carbon coupling constants ( n J CH ; n>1) in small molecules is made. NMR experiments are classified in terms of NMR pulse scheme and cross-peak nature. A discussion about simplicity, general applicability and accuracy for each...	nmrlearner	Journal club	0	07-20-2013 11:27 AM
Dynamic Nuclear Hyperpolarization in Liquids From The DNP-NMR Blog: Dynamic Nuclear Hyperpolarization in Liquids Günther, U., Dynamic Nuclear Hyperpolarization in Liquids, in Modern NMR Methodology, H. Heise and S. Matthews, Editors. 2013, Springer Berlin Heidelberg. p. 23-69. http://dx.doi.org/10.1007/128_2011_229	nmrlearner	News from NMR blogs	0	07-18-2013 12:39 AM
[NMR paper] Biomolecular magic-angle spinning solid-state NMR: recent methods and applications. Biomolecular magic-angle spinning solid-state NMR: recent methods and applications. Related Articles Biomolecular magic-angle spinning solid-state NMR: recent methods and applications. Curr Opin Biotechnol. 2013 Mar 4; Authors: Goldbourt A Abstract The link of structure and dynamics of biomolecules and their complexes to their function and to many cellular processes has driven the quest for their detailed characterization by a variety of biophysical techniques. Magic-angle spinning solid-state nuclear magnetic resonance spectroscopy...	nmrlearner	Journal club	0	03-14-2013 10:05 PM
Methods to determine slow diffusion coefficients of biomolecules. Applications to Engrailed 2, a partially disordered protein Methods to determine slow diffusion coefficients of biomolecules. Applications to Engrailed 2, a partially disordered protein Abstract We present new NMR methods to measure slow translational diffusion coefficients of biomolecules. Like the heteronuclear stimulated echo experiment (XSTE), these new methods rely on the storage of information about spatial localization during the diffusion delay as longitudinal polarization of nuclei with long T1 such as nitrogen-15. The new BEST-XSTE sequence combines features of Band-selective Excitation Short-Transient (BEST) and XSTE methods. By...	nmrlearner	Journal club	0	05-24-2011 10:00 AM
[NMR tweet] Nuclear Magnetic Resonance Spectroscopy: An Introduction to Principles, Applications, and Experimental Methods: http://amzn.to/ifB9C2 Nuclear Magnetic Resonance Spectroscopy: An Introduction to Principles, Applications, and Experimental Methods: http://amzn.to/ifB9C2 Published by Ileentxw (Ileen Basques) on 2011-04-15T14:08:05Z Source: Twitter	nmrlearner	Twitter NMR	0	04-15-2011 02:52 PM
[NMR tweet] http://www.pulist.net/nuclear-magnetic-resonance-spectroscopy-an-introduction-to-principles-applications-and-experimental-methods.html # http://www.pulist.net/nuclear-magnetic-resonance-spectroscopy-an-introduction-to-principles-applications-and-experimental-methods.html # Published by pulistbook (pulist books) on 2011-01-09T16:15:12Z Source: Twitter	nmrlearner	Twitter NMR	0	01-09-2011 04:27 PM
Using NMR to study fast dynamics in proteins: methods and applications. Using NMR to study fast dynamics in proteins: methods and applications. Related Articles Using NMR to study fast dynamics in proteins: methods and applications. Curr Opin Pharmacol. 2010 Oct 6; Authors: Sapienza PJ, Lee AL Proteins exist not as singular structures with precise coordinates, but rather as fluctuating bodies that move rapidly through an enormous number of conformational substates. These dynamics have important implications for understanding protein function and for structure-based drug design. NMR spectroscopy is particularly well...	nmrlearner	Journal club	0	10-12-2010 02:52 PM
ICMRBS founder’s medal 2006: Biological solid-state NMR, methods and applications ICMRBS founder’s medal 2006: Biological solid-state NMR, methods and applications Marc Baldus Journal of Biomolecular NMR; 2007; 39(1); pp 73-86 Abstract: Solid-state NMR (ssNMR) provides increasing possibilities to study structure and dynamics of biomolecular systems. Our group has been interested in developing ssNMR-based approaches that are applicable to biomolecules of increasing molecular size and complexity without the need of specific isotope-labelling. Methodological aspects ranging from spectral assignments to the indirect detection of proton–proton contacts in...	Deano	Journal club	0	08-14-2008 10:08 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off